在製備大面積一微奈米結構的技術中，使用DNA溶液進行圖案式分子梳是一個相當簡單、快速且便宜的方法，透過這個方法可以得到相對大面積、整齊排列的DNA奈米束陣列。以往的研究中均以手動操作的方式拉伸DNA奈米束，人為的因素可能影響製備的結果，因此本研究使用可控制轉速的旋轉塗佈機，並藉以不同尺寸、形狀的流道輔助，嘗試在具有微孔洞與微柱陣列的基板上製備DNA奈米束陣列。由實驗結果得知，使用開放的微孔洞陣列基板拉伸DNA奈米束，雖然無法控制其分佈範圍與方向性，但以離心力的方式所拉伸得到的DNA奈米束長度較以手動操作的方式拉伸來得長。在使用流道輔助製備DNA奈米束陣列時，以密閉式流道搭配微孔洞陣列基板進行DNA奈米束拉伸，可以成功製備出大面積、方向單一的DNA奈米束陣列，而使用兩端開口流道作為輔助時，則會在流道末端產生一個沒有DNA奈米束分佈，或是DNA奈米束分佈雜亂的空白區域。此外，以密閉式流道搭配微柱陣列基板進行拉伸時，若僅填充溶液於微柱頂部的區域，可拉伸出若干長DNA奈米束；若將微柱間隙填滿溶液，則得到DNA溶液所形成的圓點陣列。

Among the techniques for fabrication of 1-D micro/nanostructures on a large area, patterned molecular combing is a relatively simple, fast and low cost method. By utilizing this method, the aligned and ordered DNA nanostrand array can be generated. This method has been operated manually, which could affect the final results due to uncontrollable factors. In this study, we intend to use the spin coater and the cover plate having the channel with different dimensions and geometric shapes to generate DNA nanostrands on microwell and/or micropillar array. The results showed that, by applying the centrifugal force, longer DNA nanostrands were generated on the polydimethyl siloxane (PDMS) stamp without cover plate compared to those by manual operation. However, the distribution of these DNA nanostrands is random and they are not well aligned. When using the PDMS stamp with microwell array and cover plate with polygon-shaped reservoir and capillary valve, the aligned and ordered DNA nanostrands were generated. For using the PDMS stamp with micropillar array, either the DNA nanostrands were generated sparsely on the substrate or DNA nanodots on the top of micropillars.

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