本文針對具導電性聚仳咯(Ppy)葡萄糖生物感測器進行研究，此感測器之製備程序係在定電流情況下，以白金工作電極置入含葡萄糖氧化酵素(GODx)及仳咯單體之氯化鉀溶液中進行電聚合固定化反應。
對不同條件下所製備生物感測器的表現，本研究均予檢測並相互比較，進而獲得定電流法製備聚仳咯(Ppy)葡萄糖生物感測器的準則。本文共探討了五個影響Ppy/GODx生物感測器性質的製備因素，即；單體濃度、施加電流密度、生成膜厚度、酵素濃度、系統pH值。根據實驗結果得知：單體濃度太高或施加電流密度太小及生成膜的厚度太厚，這些條件導致感測器的靈敏度很差，不適合製備Ppy/GODx生物感測器，同時，就靈敏度而言；酵素濃度於0.5mg/mL與製備系統於微酸性及中性情況下有最佳化的情況出現。除了靈敏度以外；決定製備定電流GODx/Ppy生物感測器之最佳化條件還得考慮感測器的選擇性，而本文發現以聚仳咯作為生物感測器之酵素固定化基材時，生物感測膜之厚度只要超過50 mC/cm2
，便具有優異分子篩特性，可排除維生素C所引起的干擾問題，增加GODx/Ppy生物感測器的選擇性。實驗結果也顯示此種最佳化生物感測器在感測葡萄糖時，所得信號在0~10 mM範圍具有良好的線性關係，此線性關係亦符合理論之推導。同時；此最佳化生物感測器的酵素動力學參數，KM’及Imax可藉Lineweaver-Burk作圖法分別求出。針對定電位法所製備之GODx/Ppy生物感測器感測前之穩定化耗時作業問題，經研究後，發現定電流製備法可顯著縮短GODx/Ppy生物感測器的穩定化作業時間。最後，此最佳化生物感測器經試驗後，約具2週的長時安定性。
本文之貢獻在於：(1)建立適當條件的設計準則，製備定電流法GODx/Ppy葡萄糖生物感測器，(2)成功地以光學顯微鏡觀察到微米級固定化酵素在電極表面上之分佈情況，(3)在定電流聚合時，藉著應答電位之上升作用，提早將生物膜中之聚仳咯氧化而去其活性，如此，定電流法所製備之酵素電極可有效地縮短感測前之穩定化時間。

The polypyrrole (Ppy) glucose biosensor fabricated by galvanostatic electropolymerization of pyrrole monomer in the presence of glucose oxidase (GODx) in a neutral saline solution with a platinum electrode is reported.
The performances of the biosensors prepared under various conditions were examined and compared with each other, thus the criteria for designing the galvanostatic GODx/Ppy glucose biosensor were obtained. There were five fabricating parameters to be investigated how the effects of them on the sensing properties of the resulted glucose biosensors were. Based upon the experimental results, higher monomer concentration、lower applied current density and higher film thickness always resulted in a poor sensitivity to glucose and were unsuitable to fabricate the GODx/Ppy biosensor. Enzyme-loading amount of 0.5 mg/mL and the fabricating system with slightly weak acidic or neutral condition would lead an optimal sensitivity to the resulted sensors. Besides the sensitivity, the selectivity should also be considered for determining the optimal conditions for fabricating the glucose biosensor. The results confirmed that the polypyrrole-based biofilm with thickness higher than 50 mC/cm2 could perform the excellent size-exclusion effect to suppress the interference aroused by the model electroactive species, ascorbic acid, and enhance the selectivity of the resulted sensor. The linear range for sensing glucose of this optimized biosensor was from zero to 10 mM and in agreement with the theoretical derivative. Meanwhile, the kinetic parameters of the resulted biosensor, KM’ and Imax could be readily derived from the Lineweaver-Burk plot, respectively. As regards the time-consuming problem for stabilizing the potentiostatic GODx/Ppy bioelectrode before sensing glucose, this galvanostatic method has been proved to effectively improve this demerit. Finally, the long-term stability of this optimized glucose biosensor was also reported to be about 2 weeks.
The contributions of the present work included that establishing the criteria for designing a galvanostatically fabricated GODx/Ppy biosensor, successfully observing the spatial distribution of the immobilized commercial enzyme with m-level by a convenient optical microscope, and significantly shorting the time for stabilizing this sensor with an increasing potential response, which could previously deactivate the electroactivity of Ppy during the immobilization process before sensing glucose.

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