本論文提出利用奈米轉印技術可以簡單且低成本的製作具可壓縮狹縫之奈米雙菇結構。結合了狹縫共振與法諾共振，我們提出了具備高FOM值且同時具備侷域性電場的偵測器，用以解決以往的研究中無法良好運用偵測器來進行非標籤分子的偵測。而為了有更好的量測品質，我們也利用了嚴格耦合波分析法來針對結構的參數進行調整，最終選定狹縫為20 nm和長寬比為1.67的奈米雙菇結構，並將其應用於折射率偵測及非標籤分子偵測。
膀胱癌近年來為國內男性罹患癌症的前十名，然而現今在診斷膀胱癌的技術多以膀胱鏡為主，但膀胱鏡的診斷屬於侵入式的偵測，除了膀胱鏡的侵入所造成的不適感外，在診斷後也可能引起患者尿路的感染風險。除了膀胱鏡檢測外，也有核磁共振等較為昂貴的醫療設備可以進行診斷，但這類設備最大的問題便是其耗時且昂貴導致難以廣泛利用，因此如何開發便宜且非侵入式的偵測系統就顯得相當重要。
近年來有其他研究指出在膀胱癌患者的尿液檢體中具有可以針對膀胱癌進行辨別的指標分子玻尿酸(hyaluronic acid，HA)及玻尿酸酶(Hyaluronidase，HAase)，透過對患者尿液中的指標分子含量分析來達到膀胱癌的診斷。目前本研究所提出的奈米雙菇結構已能在偵測0.5 mg/mL的HA檢體中能得到6 nm的訊號變化；在偵測400 U/mL HAase也能有17 nm的訊號變化，在開發膀胱癌的非侵入式的偵測系統中具有相當大的潛力。

Surface Plasmon Resonance (SPR) sensor have been widely used for chemical and biological sensing due to the high sensitivity to the refractive index of the dielectric environment. Previously, a lot of research indicated plasmonic structure with Fano resonance for enhancing the FOM. However, it can’t have the producing results in label-free sensing after having this reply. The reason is that localized electric field distribution cannot be generated for label-free sensing. Therefore, we proposed the nano dimer-mushroom array (NDMA) for the sensor with the nanogap for producing the localized electric field distribution and the coupling between resonances for enhancing the FOM. Its sensitivity can reach 640.32 nm/RIU and the FOM can be boosted up to 49.3. In label-free sensing, we investigated HA and HAase, the biomarkers of bladder cancer, with 6 nm and 16 nm resonance shift.

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