本論文主要探討如何運用一個新的微流體平台，來實現各種電荷動力方法操控單分子DNA，本實驗利用兩相不互溶的流體在微流道中產生一個扁長型液珠使其大小幾乎貼近微流道管壁，而另一相流體則存在於此液珠與管壁表面之間形成了一個具有侷限效應的界面薄膜，其阻礙效應可迫使長鏈DNA分子產生形變。
在外加電場的作用下配合此薄膜效應，使本實驗不僅實現了DNA分子之熵致捕捉、拉伸行為、以及DNA分子由油珠入口鑽入薄膜內的動態行為，更發現了DNA受到玻璃表面的界面活性劑影響，造成一種類似船錨般鉤附在界面活性劑疏水端的現象，在電場的作用下DNA在表面上進行一連串短暫接觸、拉伸、脫附、收縮的現象，因為DNA隨著其分子大小，與界面活性劑接觸的程度不同，所以不同DNA分子其遷移率隨著電場改變的程度也不同，因此提供了一個可用來分離DNA的可能方法。除此之外當玻璃表面上界面活性劑的吸附密度增加時，更形成了DNA分子梳，除去電場後，DNA分子間受到遠距的絮凝作用，而在表面上形成多個DNA分子聚集團，以至於吾人能夠觀察到DNA自身的組織行為，爾後更因為一開始並排拉伸的DNA在收縮鬆弛的過程中有橫向交聯的原故，使多個DNA分子聚集團在平行於電場的方向形成DNA分子束。除此之外我以直流電將DNA注入底層薄膜後，改以交流電場作用，發現DNA於油珠底層薄膜內存在一淨速度往其載入的反方向移動，更有類似大分子DNA在凝膠中延展纏繞於孔間隙的情形。以上各種DNA操控皆能在此次微米薄膜平台內達成。

This thesis focuses on a new microfluidic platform capable of achieving a diversity of electrokinetic manipulations of DNA at the single-molecule level. The strategy invokes a submicron film between a closely fitting slug of the second fluid and the channel surface, creating an interfacial confinement to render conformation changes of a flexible DNA chain. I not only demonstrate that DNA molecules within the film can undergo entropic trapping, stretching, and translocation under the actions of an electric field, but also find the unique cyclic stick-slip behavior of the DNA motion with field and size dependent mobility due to interim anchoring between the DNAs and the surface surfactants, which has potential applications to on-chip DNA separation. In addition, with a dynamic template created by dense surface surfactant crowds, molecular combing and self organization of elongated DNA chains are also illustrated for the first time. In the latter case, DNAs can exhibit long-range flocculation or even develop to mesoscale bundles by the crosslink between the stretched chains. I also observe how DNAs respond to ac fields after being injected into the film with a dc field. I find, curiously, that the DNAs not only exhibit net motion toward the injection end but also show reptation behavior during its journey.
All these findings suggest new paradigms for controlling the motion of DNA within submicron confinement.

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