本研究利用多巴胺結合金與氧化鐵粒子合成金奈米氧化鐵材料，合成過程中不需額外加入化學品且耗費的時間僅需10分鐘，金奈米氧化鐵材料在750 nm處具有增近紅外光吸收之特性，且結合三種過去發現的光熱材料並達到更好的光熱轉換效率，除了光熱方面的應用還可利用於檢測細菌，透過表面增強拉曼光譜來辨別細菌的存在，而傳統檢測方式可分為計數法、顯微鏡檢法、冷光量測法，但這些方法需要耗費時間及人力資源且量測準度會因處理時間上升而降低，本研究利用近1W紅外雷射激發50 ppm的金奈米氧化鐵材料，可使材料升溫到60 °C並具備光熱升溫殺菌之效果，同時收集樣品產生的拉曼光譜，即可透過訊號來判別細菌的存在，且利用材料本身的磁性達到回收再利用的效果。

The integration of magnetic and optical functions with nano-Fe3O4 and nano-Au showed mag-netic-guided concentration to improve the signal responsive sensitivity of surface enhanced Raman scattering (SERS) and the photothermal conversion efficiency to track and treat cancer cells. Here-in,we utilized L-dopamine molecules as a buffer layer to spontaneous growth of Au shell around the Fe3O4 nanoclusters. After the generation of Au-Fe3O4 nanohybrid, the specific surface plasmon resonance peak at ~ 750 nm appeared that the peak position was depended on the synthesis param-eters, i.e., reagent concentrations and reaction temperatures. We analyzed the SERS effect (with a micro-Raman using a 785 nm laser) of Au-Fe3O4 nanohybrid decorated with 4-Aminothiophenol, displaying strong 4-fold signal intensity enhancement at 1071cm-1 and 1572 cm-1 after magnetic concentration as compared to magnetic free Au-Fe3O4 nanohybrid. As subject the Au-Fe3O4 nano-hybrid to 808 nm laser light, a particle-including solution temperature increased from 27 °C to the 60 °C. Such temperature could be applied to photothermal destruction of HeLa cancer cells. In ad-ditional, we demonstrated that the Au-Fe3O4 nanohybrid is useful label-free material to distinguish the optical fingerprint Escherichia coli and Staphylococcus aureus by using SERS spectra. As ex-posure of particle-treated microbe to 808 nm laser after collection with a magnet, the anti-bacteria experiment showed ~99% killing rate for 1x105 cfu/ mL of E. coli and S. aureus with Au-Fe3O4 na-nohybrid (50 ppm[Fe]).

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