本論文係有關聚丙烯酸(PAA)與幾丁聚醣(Chitosan)被覆之氧化鐵磁性奈米粒子的製備及其作為磁性奈米吸附材料應用於吸附伴刀豆球蛋白的研究。
關於被覆PAA之磁性奈米粒子的製備，首先以化學共沈澱法製備出Fe3O4磁性奈米粒子，然後將PAA藉碳二醯胺活化直接共價鍵結在磁性奈米粒子上，使磁性粒子表面帶有羧基，形成具有離子交換功能之磁性奈米載體，並藉由穿透式電子顯微鏡(TEM)、X射線繞射儀(XRD)得知磁性奈米粒子在共價鍵結PAA後，其大小、結構並無明顯改變。由傅立葉轉換紅外線光譜儀（FTIR）、熱重分析儀(TGA)、熱差分析儀(DTA)與光學分析法分析可確認PAA已共價鍵結在磁性奈米粒子上。此外，也以界面電位(Zeta potential)儀量測粒子表面帶電特性的變化。當以被覆PAA之磁性奈米粒子吸附伴刀豆球蛋白時，發現吸附平衡所需時間約為40分鐘，且在pH4的磷酸鹽緩衝溶液中具有最佳的吸附效果。而由動力學數據得知此吸附行為接近擬二階吸附模式，且無內部擴散阻力存在。又當鹽類濃度上升時，蛋白質吸附量會減少，且藉溶液pH變化與提升鹽類濃度進行脫附，均可於20分鐘以內達到脫附平衡，且脫附比率分別為80％與30％。此外，恆溫吸附曲線顯示吸附行為符合Lamgumuir恆溫吸附模式，其最大吸附容量(qm)和Langmuir平衡常數(KL)分別為175.33mg/g與3.95ml/mg。當使用磁性奈米粒子吸附白鳳豆萃取液時，由於PAA對蛋白質並無特異性作用力，故雖能吸附白鳳豆中伴刀豆球蛋白，但選擇性不高。
關於幾丁聚醣被覆之氧化鐵磁性奈米粒子的製備，係先將幾丁聚醣羧基甲基化，再藉由碳二醯胺的活化將羧基甲基化幾丁聚醣共價鍵結在氧化鐵奈米粒子上，然後利用穿透式電子顯微鏡、X射線繞射光譜儀、傅立葉轉換紅外線光譜儀、熱重分析儀、熱差分析儀與光學分析法分析可確認所得產品的特性。當以被覆幾丁聚醣之磁性奈米粒子吸附伴刀豆球蛋白時，發現吸附平衡所需的時間約為30分鐘，且在pH5的磷酸鹽緩衝溶液中具有最佳的吸附效果，並推測氫鍵為主要的作用力。此外，也探討螯合重金屬之幾丁聚醣磁性奈米粒子吸附伴刀豆球蛋白的行為。發現被覆幾丁聚醣之磁性奈米粒子螯合Ni(II)與Co(II)之後，對蛋白質吸附量有明顯的抑制效果，而在螯合Cu(II)之後則無明顯的影響。

This thesis concerns the preparation of polyacrylic acid (PAA) bound and chitosan bound iron oxide magnetic nanoparticles and their use for the adsorption of Concanavalin A (Con A).
For the preparation of PAA bound iron oxide magnetic nanoparticles, Fe3O4 magnetic nanoparticles were synthesized by the coprecipitation method first. Then, PAA was covalently bound onto magnetic nanoparticles via carbodiimide activation to form the magnetic nano-carrier with carboxylic groups for ion exchange. Transmission electron microscopy (TEM) and X-ray diffraction (XRD) analyses revealed that the PAA binding did not result in the significant changes in the size and structure of Fe3O4 cores. The binding of PAA on magnetic nanoparticles was demonstrated by the observation of Fourier Transform Infra Red (FTIR) spectroscopy, thermogravimetric analysis (TGA), differential thermal analysis (DTA), and spectrophotometric assay (OPA method). The surface charge was measured by a zeta potential meter. Using PAA bound magnetic nanoparticles to adsorb Con A, it was found that the time required to achieve the adsorption equilibrium was about 40 min and a maximum adsorption capacity was obtained in the phosphate buffer at pH 4. The kinetic adsorption data revealed that the adsorption process could be described by a pseudo second-order model equation and had no internal diffusion resistance. Also, the adsorption capacity decreased with the increase in the salt concentration. By varying the solution pH or increasing the salt concentration, the desorption equilibrium of Con A could be achieved in 20 min. In addition, the adsorption data obeyed the Langmuir isotherm equation. The maximum adsorption capacity and equilibrium constant were found to be 175.33mg/g and 3.95ml/mg, respectively. Because PAA has no specific interaction with Con A, the PAA bound magnetic nanoparticles could adsorb effectively Con A from Jack Bean extract but the selectivity was low.
For the preparation of chitosan bound iron oxide nanoparticles, chitosan was carboxymethylated first and then covalently bound onto the surface of iron oxide nanoparticles via carbodiimide activation. The product was characterized by TEM , XRD, FTIR spectroscopy, TGA, DTA, OPA method, and zeta potential. Using chitosan bound magnetic nanoparticles to adsorb Con A, it was found that the time required to achieve the adsorption equilibrium was about 30 min and the maximum adsorption capacity was obtained in the phosphate buffer at pH 5. It was suggested that the main interaction was hydrogen bonding. In addition, investigating the adsorption behaviors of Con A by the metal ions chelated chitosan bound magnetic nanoparticles, it was found that the adsorption capacity increased slightly for Cu(II) chelated chitosan bound magnetic nanoparticles but significantly decreased for Ni(II) or Co(II) chelated chitosan bound magnetic nanoparticles.

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