Dipalmitoyl phosphatidylcholine（DPPC）為肺泡界面活性劑中降低肺泡內襯液層表面張力的主要成分，但因DPPC價格昂貴，所以本實驗利用結構與DPPC相似的hexadecyltrimethylammonium-hexadecylsulfate（HTMA-HS）進行單分子層實驗。結果顯示崩潰表面壓能達到72.0 mN/m，代表此時界面上的表面張力接近零。由上述結果推測HTMA-HS有取代DPPC作為肺泡界面活性劑之主要物質並用於治療呼吸窘迫症的潛力。但在牛血清白蛋白（bovine serum albumin, BSA）吸附分子層存在下於氣/液界分佈HTMA-HS單分子層，連續來回壓縮-擴張界面，發現HTMA-HS分子明顯損失，應是HTMA-HS分子容易隨albumin離開氣/液界面，使得HTMA-HS的動態界面活性受到抑制。
本研究以1,2-二棕櫚醯基-sn-甘油-3-磷酸乙醇胺-N-[（甲氧基）聚乙烯醇-550]（DPPE-PEG550）做為添加劑，來改善HTMA-HS易與albumin作用而從氣/液界面上損失的情形。結果顯示隨DPPE-PEG550添加比率上升，DPPE接枝的親水性高分子PEG影響混合單分子層的排列造成表面較不平坦，使得在壓縮過程中混合分子更容易從界面上脫附，導致分子損失率上升。但不管添加1、3或10 mol%的DPPE-PEG550都無法有效減少HTMA-HS單分子層的損失率。比較HTMA-HS/DPPE-PEG550/albumin和文獻中HTMA-HS/DPPE-PEG1000/albumin、HTMA-HS/DPPE-PEG2000/albumin混合系統的結果，顯示當PEG鏈愈長時，albumin在擴張過程中愈不易再吸附到界面上，對albumin有愈大的排斥效應或產生愈顯著的立體障礙。但較長PEG鏈的溶解度較大，對混合單分子層的排列影響較大，使混合單分子層在壓縮過程中容易脫附，可是較不平坦的表面會使albumin不易再吸附到界面上，且當albumin靠近時，對其產生的排斥行為也愈顯著。由上述的結果可以歸納出DPPE-PEG對分子損失的影響因素有溶解度、對混合單分子層排列的影響，以及對蛋白質的排斥效應或產生的立體障礙。

In this study, the effects of bovine serum albumin (BSA) on the mixed Langmuir monolayer behavior of hexadecyltrimethylammonium-hexadecylsulfate (HTMA-HS) with dipalmitoyl phosphatidylethanolamine-polyethylene glycol 550 (DPPE-PEG550) was investigated by the Langmuir trough approach. Cyclic compression-expansion isotherms of the monolayers were examined to elucidate the induced removal characteristic of the molecules at the air/liquid interface. For mixed monolayers of HTMA-HS with adsorbed BSA at the cyclic interface, HTMA-HS would leave the interface with BSA during the interface compression stage, causing the loss of HTMA-HS at the interface. The presence of DPPE-PEG550 in the mixed monolayers would affect the monolayer structure. The PEG chains were helpful in preventing the readsorption of BSA during the interface expansion stage, which could be explained by the high degree of steric exclusion associated with the segmental flexibility of PEG chains.

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