本研究以Poly(L-lysine) (PLL) (Mw = 97800, 40600, 19100) 為骨幹與酰基酸酐反應合成Poly(L-lysine)-graft-decanoyl (PLD) 和 poly(L-lysine)-graft-tetradecanoyl (PLT) ，並探討其自組裝行為。從實驗結果發現藉由調控酰基上的碳鏈長度和接枝比例不但會改變雙親性高分子的親疏水特性，亦能改變聚胺基酸的二級結構並進而影響自組裝粒子的大小及構型。在酸性和中性條件下所製備的奈米微胞大小由動態光散射 (DLS) 及穿透式電子顯微鏡 (TEM) 證實約 70 至 235 nm，而臨界聚集濃度 (CAC) 在 5.2×10-2~1.0×10-3 mg/mL。此結果與先前所探討 poly(L-lysine)-graft-hexanoyl (PLH) 的粒徑和 CAC 還來的小以外，自組裝構型也不同。藉由微胞的內核疏水特性包覆疏水性天然藥物-薑黃素，經由 DLS測得粒徑約在 90 至 165 nm，並定出包覆薑黃素的效率可達 43 % 。更甚，將此可生物相容性、生物可降解性的藥物載體奈米粒子中加入槴仔素 (Genipin) 以穩定其結構，粒徑範圍在 100 至 190 nm。透過一連串對聚胺基酸上的官能基修飾所得到的 PLD 和 PLT 不僅能夠藉由環境應答改變奈米粒子的大小，亦能裝載疏水藥物，使這些兩親性共聚胺基酸在未來能夠有機會應用於應依領域的標靶藥物載體或奈米包覆體。

The simple preparation strategy and self-assembly behavior of poly(L-lysine)-graft-decanoyl (PLD) and poly(L-lysine)-graft-tetradecanoyl (PLT) have been demonstrated. In the study, we synthesized three different poly(L-lysine) (PLL) chain length (Mw = 97800, 40600, 19100) and incorporated with two different acyl chain in various substitution levels by reacting with acyl anhydride. The experimental data revealed that the interplay between hydrophobic fatty chain and the secondary conformational changes determined the self-assembed nanostructures of PLD and PLT. The micellar nanostructures were formed with the mean sizes between 70 and 325 nm in acidic and neutral aqueous solution. The critical aggregation concentration (CAC) were in the range of 5.2×10-2~1.0×10-3 mg/mL, which is smaller than our previous report on poly(L-lysine)-graft-hexanoyl (PLH). The chain conformational changes were observed the increase of relative content of helix, sheet and turn conformations as a result of incorporation of acyl chains and different pH value. The as-prepared particles were employed for natural hydrophobic drug encapsulation. The curcumin were used as a model drug with the encapsulation efficiency higher than 90% and loading capacity up to 43%, which is higher than most of the related studies. The sizes of curcumin-loaded micelles were range between 90 and 165 nm, and would increase to 100~190 nm when further crosslinking by genipin. With versatility of this synthesis strategy, additional functionality can be incorporated on PLD and PLT, which can allow these amphiphilic copolypeptides to be useful as targeted drug carriers, nanoencapsulants in the biomedical fields.

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