由於人體軟骨組織的缺損或變異，使得臨床上出現許多的疾病或功能與外觀的喪失，進而影響生活品質。例如，退化性關節炎，造成每年數萬人必須接受關節置換手術;先天性小耳症，造成先天的耳朵畸形而影響外觀與社交品質。由於軟骨組織生長的環境特殊，較少有血管支配以提供養分，處在相對缺氧的環境，相對的代謝與吸收的速度較低。加上有彈性、耐磨擦的特性，因此能長時間維持一定的體積。然而，軟骨細胞不易生長，且在體外培養容易老化且失去原有軟骨細胞的特性，且軟骨組織不易建造的原因，因而造成軟骨缺損修復不易。許多的研究與策略都不斷的被嘗試著，包含利用有潛能的幹細胞，如骨髓間質幹細胞，脂肪幹細胞等，單獨或合併不同來源的細胞骨架或載體來修復，或建造軟骨組織。但目前應用較為廣泛，成熟且可靠的細胞型態，仍以成熟的自體軟骨細胞為主要的應用對象。由於它的來源受限，且不易體外大量培養，因此有其侷限與限制。若應用幹細胞作為細胞的來源，則又有不易誘導成軟骨的限制。軟骨膜前驅細胞來自耳朵，位於身體表層，較容易取得且傷害較小，不會造成耳朵軟骨外觀的變形。本篇的論文研究主題，希望了解軟骨膜前驅細胞，並希望利用軟骨膜前驅細胞作為軟骨新生或缺損修復的細胞來源，作為日後軟骨新生或軟骨缺損修復的重要角色。

The defect or variation of cartilage tissue has many clinical consequences, resulting in poor quality of life. For example, degenerative arthritis causes tens of thousands of people to undergo joint replacement surgery every year; congenital microtia, whose congenital ear deformities and affects the appearance and social quality. Due to the special growth environment of cartilage tissue, it is dominated by fewer blood or blood vessels, in a relatively hypoxic environment, and the relative metabolism and absorption rate is low. Therefore, it is elastic, abrasion-resistant, and can maintain a certain volume for a long time. However, chondrocytes are not easy to grow, and it is easy to age and lose the characteristics of original chondrocytes when cultured in vitro, which causes difficulty in repairing cartilage defects and the reasons that cartilage tissue is not easy to build. Many kinds of research and strategies are constantly being tried, including the use of potential stem cells, such as bone marrow mesenchymal stem cells, adipose stem cells, etc., alone or in combination with cytoskeletons or carriers from different sources to repair or build cartilage tissue. However, the currently more widely used and reliable cell types are still dominated by mature autologous chondrocytes. Because of its few sources, it is not easy to cultivate in large quantities in vitro, so it has its limitations and limitations. Perichondrial progenitor cells come from the ear and are located on the surface of the body. They are easier to obtain and do not cause damage to the ear cartilage. The research theme of this paper hopes to use perichondrial progenitor cells as one of the cell sources for cartilage regeneration or cartilage defect repair, as an important role in cartilage regeneration or cartilage defect repair in the future.

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