本研究以壬醛修飾的疏水改質聚核苷酸結合離子對雙親分子（ion pair amphiphile, IPA）製備的陰陽離子液胞形成複合物，並進行相轉移溫度、穩定性及釋放行為等測試，評估其作為藥物傳輸載體的應用潛力。實驗結果顯示，帶負電的疏水改質聚核苷酸能藉靜電吸引力吸附於帶正電的液胞表面形成穩定複合物。比起純液胞組別，該系統具有更低的相轉移溫度，推測與改質聚核苷酸結構中的疏水碳鏈插入液胞雙層膜結構、使膜流動性提升有關。接著評估複合物應用於人體的可能性，發現其在類生理的酸鹼環境(pH 6.8、7.4)中展現極好的穩定性，至於不同離子強度下則會因為電雙層壓縮等原因讓其產生輕微聚集的現象。最後，藥物釋放行為數據顯示，結合改質或未改質聚核苷酸的液胞複合物，表面緻密高分子吸附層提供了質傳阻力，降低藥物通過雙層膜到達外部介質的速率，因此表現出相對純液胞更緩慢的釋放行為；而液胞/疏水改質聚核苷酸複合物釋放速率又略高於未改質組別，推測與疏水改質碳鏈插入雙層膜後提升膜流動性有關。綜合研究結果，疏水改質聚核苷酸結合液胞後可同時有效調控液胞雙層膜性質與釋放行為，並且其在類生理環境下展現相對純液胞系統更佳的穩定性，顯示其良好的應用潛力。

To address the challenges in the stability and controlled release of nanoscale drug carriers, this study investigates the potential of complexes formed by catanionic vesicles and hydrophobically modified polynucleotides (PN-R) as drug delivery systems. The negatively charged modified polynucleotides electrostatically adsorb onto positively charged vesicles, resulting in stable complex formation. Compared to the unmodified group, the vesicle/PN-R complexes exhibit a reduced phase transition temperature, likely due to the insertion of hydrophobic side chains into the bilayer, which enhances membrane fluidity. The vesicle/PN-R complexes demonstrate excellent stability under physiologically relevant pH conditions (pH 6.8 and 7.4), while slight aggregation is observed under high ionic strength (0.15 M), potentially due to electrical double layer compression. Drug release studies indicate that complexes with polymer coatings exhibit slower release profiles relative to bare vesicles, attributable to the presence of a dense polymer layer acting as a diffusion barrier. Notably, complexes with hydrophobically modified polynucleotides show a slightly faster release rate than their unmodified counterparts, which may be associated with increased bilayer fluidity. Overall, the results suggest that hydrophobically modified polynucleotides can effectively modulate bilayer properties and release behavior when incorporated into vesicle systems, while also enhancing colloidal stability under physiological conditions, underscoring their promise for drug delivery applications.

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