本研究的目的是開發類乙醇體陰陽離子液胞(ethosome-like catanionic vesicle)作為經皮藥物傳輸的載體。首先使用沉澱法製備三種不同碳鏈長度的類脂質離子對雙親分子(ion-pair amphipile, IPA)，分別為DeTMA-DS、DeTMA-TS以及DTMA-DS。然後透過半自發製程，在含20%乙醇的緩衝溶液中製備三種類乙醇體陰陽離子液胞，用以包覆水溶性藥物熊果素(Arbutin)。並進一步探討膽固醇的添加對類乙醇體陰陽離子液胞的物理特性和藥物包覆效率之影響。此外，也探討膽固醇濃度及溫度對藥物釋放行為的影響。
實驗結果顯示，膽固醇的添加對初始粒徑及介面電位之影響並不顯著，但是對管柱分離後液胞粒徑則會有不同的結果。對DeTMA-DS與DeTMA-TS兩個系統，分離後粒徑隨膽固醇濃度增加而增大，而DTMA-DS系統無明顯變化。此結果與膽固醇濃度對包覆效率的影響呈現正相關，但與液胞雙層膜剛性則無一致的關聯，進而推論主導水溶性藥物包覆效率的因素為液胞粒徑，而非雙層膜剛性。溫度上升都會使藥物釋放速率增快；而膽固醇對釋放速率的影響則隨著溫度高低而有不同的結果。在凝膠相(gel phase)添加膽固醇會使其釋放速率加快；在液晶相(liquid-crystalline phase)添加膽固醇會使其釋放速率減緩，推論原因與膽固醇對不同相態的雙層膜剛性有相反作用所致。

This study aimed at developing ethosome-like catanionic vesicles for transdermal drug delivery. Three ion-pair-amphiphiles, were prepared by precipitation method. They were DeTMA-DS, DeTMA-TS and DTMA-DS, respectively, and were thereafter used as the raw materials for preparing stable ethosome-like catanionic vesicles with the aid of cholesterol and ethanol in aqueous buffer solution whose pH value was 7.4 by the semispontaneous process. The potential applications of the ethosome-like catanionic vesicles as nano-carriers in dermal drug delivery were demonstrated to study cholesterol and temperature effects by release behavior of Arbutin, a water-soluble drug. Furthermore, effects of cholesterol on physical properties and encapsulation efficiency. Experimental results showed that the vesicle size in DeTMA-DS and DeTMA-TS systems were increased, and in DTMA-DS system didn’t change obviously. These results were similar with encapsulation efficiency. Vesicle size was a dominant factor on encapsulation of water-soluble drugs. In the parts of drug release, when the temperature from 20°C to 40°C would make the release rate fast. However, cholesterol caused opposite effects on the release rate for vesicular bilayers at different phases. While release rate was increased with the increase of cholesterol concentration for bilayers at gel phase, release rate was decreased with the increase of cholesterol concentration for bilayers at liquid crystalline phase. The opposite cholesterol effects on release rate were explained by the disordering and condensation effects, respectively, on the rigidity of vesicular bilayers.

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