本研究以光散射實驗分析電紡絲液柱與內部微結構，藉聚乙烯醇與聚(異丙基丙烯醯胺)之混摻水溶液作為研究主體，此混摻水溶液在室溫時有相分離domain，藉由升降溫只能觀察到在15 oC時相分離domain邊界隨溫度改變而漸不明顯，推測是因為polymer rich與polymer poor兩區域的折射率差異很小。因此將藉由光散射來量測溶液於不同溫度下之光散射強度分布，並選擇以光散射強度最弱的15 oC進行後續研究。

藉雷射打擊不同之9 wt% PNIPAM/PVA混摻組成水溶液，以相同的操作參數觀察雷射打擊在穩定電紡之距離針底不同位置泰勒錐之散射圖案變化；而有關電紡液柱，將以沿著赤道方向掃描距離針底不同距離之散射圖案，將所得強度分佈中亮峰的位置，以Mie theory模擬出的強度分布做擬合，得到電紡混摻組成20/80有dj(z)~z-1.51關係；當固定雷射打擊距離針底相同泰勒錐之位置，藉改變操作電壓觀察泰勒錐與散射圖案之變化。

最後以乾玻片與液態氮收集電紡液柱，藉由OM與SEM觀察原先具相分離的溶液經電紡拉伸作用後，液柱表面或內部會存在顆粒狀與線狀微結構形態，呼應相分離domain經由電紡過程中的拉伸作用產生微結構的推測。

In this study, the electrospinning jet and internal microstructures were analyzed by light scattering experiments. The aqueous solution of PVA and PNIPAM was used for the study.
At room temperature, phase separation domains in the blend solution, and it might not be possible to find the region of one phase by changing the temperature. Therefore, light scattering was utilized to measure the intensity distribution of the solutions at different temperatures, the result indicate that the light scattering intensity is the weakest at 15 °C for subsequent investigations.

He-Ne laser showed on Taylor cone and electrospinning jet of different weight ratios 9 wt% PNIPAM/PVA blend aqueous solutions, and the scattering pattern which is at different distances from the needle end on the screen behind the jet were scanned alone the equator direction to obtain the intensity profile.Fit the position of the peak in the intensity profile with the profile simulated by Mie theory to find the jet diameter profile dj(z). The relationship of the weight ratio 20/80 can be shown as dj(z) ~ z-1.51.

Final, we collected the electrospinning jet with slides and liquid nitrogen, and observed internal microstructures with OM and SEM. The internal microstructures of electrospinning jet are presumed to be generated by the stretching effect of the electrospinning.

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