生醫材料在現今醫學應用很廣泛，但其生物相容性是個須克服的問題，因此將材料表面改質以增加生物相容性是個很重要的議題。表面改質技術中，自組裝單分子層提供了一個很好的方式，可形成緻密的排列並且末端官能基也可自由搭配以達所需的性質。本研究利用自組裝單分子層以仿生的概念將細胞膜內富含的磷脂質中的磷酸化乙醇胺官能基製成鏈長為11 碳的硫醇(11-mercaptoundecanylphosphoryl ethanolamine, HS-(CH2)11-PO4H-CH2CH2-NH3+)，將此硫醇以不同溶劑及不同濃度製備自組裝單分子層，利用表面分析儀器、電化學分析及血小板吸附的方式探討以此材料製成的自組裝單分子層，以了解不同溶劑及濃度對自組裝單分子層結構的的排列性質及血液相容性。
　　由接觸角測試的結果顯示，與未改質的金基材相比，含磷酸乙醇胺之自組裝單分子層較為親水，其中又以去離子水製備的0.1mM硫醇溶液為最佳；由化學分析電子光譜儀的結果推測，SAMs 表面因末端巨大官能基產生的立體障礙及電荷效應使得排列較差，此現象可解釋與部分接觸角偏高的現象；由S2p 光譜可看出每種條件製備的SAMs 其末端皆有未鍵結硫的產生；也可由N1s 看出有NH2 的產生。由CV 分析結果得知所形成的氧化還原峰不明顯，代表硫醇分子緊密排列在基材表面，且覆蓋很好；由EIS 分析結果所得的阻抗質皆很大，代表硫醇分子緊密排列或是材料表面帶電性的影響使得阻抗變大。
　　由血小板吸附的測試中得知，血小板在部分試片上有極度活化、聚集的情形，以血液相容性看來，含磷酸乙醇胺官能基的硫醇製備出的SAMs 血液相容性較未改質的金試片好，其中以0.1mM 去離子水製備的SAMs 血液相容性為最佳。

Biomedical materials have been widely used for medical environments, but its biocompatibility remains concerned. The modification of surface property to improve biocompatibility, therefore, has become crucial. Self-assembled monolayers (SAMs) technologies have been shown as avenues to achieve surface modification, since SAMs can be formed densely-packed while the functional group can also be tuned in accordance with different needs. This study utilizes biomimetics to synthesize 11-mercaptoundecanylphosphoryl ethanolamine (HS-(CH2)11-PO4H-CH2CH2-NH3+), one of the phospholipids abundant in cell membrane. The SAMs with different solvent and concentration are examined by surface analysis, electrochemical analysis, and platelet adhesion method, so as to understand its surface characterization and blood compatibility.
The contact angle measurements indicated that the SAMs formed by phosphorylethanolamine functional group thiol are more hydrophilic than the SAMs formed by bare gold, especially 0.1mM deionic water is used. The structural disorder of SAMs in the electrochemical analysis suggests the charge effect and steric hindrance caused by large functional group and; it is the reason why the contact angels are higher. The ESCA data demonstrates clearly the unbound thiol in S2p spectra, and NH2 peak in N1s spectra. The CV data shows unapparent oxidized-redox peak, which denotes that PE SAMs are formed closed-packed and fully covered, meanwhile, the huge impedance caused by PE SAMs closed-packed or charged surface also appears in the EIS analysis. Furthermore, the platelet adhesion exhibits platelet activation and aggregation in some samples. On the other hand, the SAMs with phosphorylethanolamine functional group performed better than the sample of gold in terms of blood compatibility, and the SAMs have the best performance when prepared by 0.1mM deionic water.

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