此研究利用高分子當作為光介電泳晶片的材料以及可撓性薄膜作為基板，並應用在相關的細胞及奈米微珠的操控，其中研究主題包含了材料光電轉換機制、晶片製程參數，及晶片效能提升。首先，文中以一個嶄新的材料及可撓式基板來操控奈米微珠，利用投射出之光線移動奈米微珠到指定之位置，並且彎曲晶片來測試不同彎曲角度對奈米微珠操控所需的光介電泳力之比較及提升。此晶片之主動層結構不是使用常見的非晶矽，而是由P3HT/PCBM所構成的異質界面高分子薄膜，利用溶液製程，將光主動層旋轉塗佈至可撓性基板上。整個實驗製程是在低溫下進行並且沒有使用非晶矽之電漿輔助化學氣相沉積之薄膜摻雜步驟。當以一投影機光源照射至單層異質界面結構之材料時，此時因電子施體(Donor)-受體(Acceptor)間之接觸面積較大，當材料吸收光能轉換為激發子(Exciton)後，能有效地被分離(dissociation)成電子與電洞，在主動層中的電子與電洞將引至不均勻電場來對細胞或微粒子進行操控。利用電腦軟體先設計好所要投影至晶片之動畫，並將之投射至可撓性異質界面高分子光介電泳晶片上，所誘發產生之虛擬電極將產生負光介電泳力，利用此現象可用來對生物體進行操控。同時也針對高分子膜厚對整體效能進行分析及研究，並且由於在配置高分子溶液時將溫度調至40 °C，故高分子膜厚可以增加至763 nm當高分子濃度是5 wt%的情況下。另外使用不同寬度、顏色、光強度之光線照射和施加之電壓於可撓性晶片，探討之效應並找出最佳實驗參數。並且探討高分子材料和不同高分子膜厚之紫外線可見光吸收光譜。彎曲可撓性晶片至凸型或凹型晶片時，能使分離不同大小尺寸之奈米微珠或收集奈米微珠之效應更有效率並且更加快速。40 μm奈米微珠在特定時間內之移動距離增加了150 %，從43.0±5.0 μm (平板晶片) 增至 112.3±3.0 μm (凸型晶片)，加上了重力的輔助會使奈米微珠沿著彎曲晶片之斜面滑落，可達到快速分離不同大小之奈米微珠或收集相同大小之奈米微珠，此可撓性異質界面高分子光介電泳晶片位生物醫學應用提供了一個理想平台。此可撓性高分子晶片可用連續捲軸式之製程和大面積塗佈之步驟並輔以噴墨壓印技術，快速產生大量成品。不僅如此，主要的便利性是低價之優點可使晶片變成可拋棄式之型態使用於生物醫學的應用上。除此之外，可撓性基板可以輕易地變換型態以達到不同型態之操控生物體之需求，因此比起非晶矽之光介電泳晶片有更多樣化的功能。

This study presents a novel technology to manipulate micro-particles with the assistance from flexible, polymer-based, optically-induced dielectrophoretic (ODEP) devices. These ODEP devices were made of a thin-film bulk-heterojunction polymer, instead of the usual amorphous silicon (a-Si), by a solution-based, spin-coating process as the photoactive layer on a flexible indium-tin-oxide/plastic substrate. A thin film of a bulk-heterojunction (BHJ) polymer, a mixture of regioregular poly(3-hexylthiophene) (P3HT) and [6,6]-phenyl C61-butyric acid methyl ester (PCBM), was used as a light-activated layer. When illuminated by a projected light beam, the photo-induced charge carriers, created by the electron transfer of excitons at a donor/acceptor interface in the BHJ layer, disturbs the uniformly-distributed electric field applied on the ODEP devices. A negative DEP force was then generated by virtual electrodes defined by the optical images generated from a computer-programmable projector to manipulate micro-particles, thus providing a flexible platform for particle manipulation. The entire fabrication was a low temperature process and no sophisticated thin-film processing techniques, such as a plasma-enhanced chemical vapor deposition step for a-Si, were required to prepare the polymer layer. Bending the flexible ODEP devices downwards or upwards to create convex or concave curvatures, respectively, enables the more effective separation or collection of micro-particles with various diameters. The travel distances of polystyrene beads with 40 μm diameters, as induced by the projected light during a given time period, was increased by ~150 %, which quantitatively were 43.0± μm and 112.3±3.0 μm for flat and convex ODEP devices, respectively. The rapid separation or collection of micro-particles can be achieved with the assistance of gravity because the falling polystyrene beads followed the inclination of the downwardly or upwardly bent ODEP devices, which could make this an ideal platform for biomedical applications. The fabrication of flexible polymer-based chips can be achieved by a continuous roll-to-roll and large-area coating processes, making them practical for mass production. Furthermore, the low-cost advantage enables the chips to be disposable, a major convenience in biological applications. The effect of the polymer thickness, applied voltage, scanning line width and scanning line color on the magnitude of the generated ODEP force in flat ODEP devices has been extensively investigated. Also the effect of scanning line width, optical power and curvature on the travel distance in curved ODEP devices was experimentally verified. The ultraviolet-visible (UV-Vis) absorption spectrum of the P3HT:PCBM film and the effect of various film thicknesses was also measured. Additionally, the flexible substrates can be easily deformed to optimally enhance and to fulfill the different manipulation requirements for micro-particles in various applications, thus demonstrating functionalities that are superior to those of a-Si-based ODEP devices.

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