龍膽石斑魚是台灣養殖業當中相當重要的經濟魚種，本研究試圖為龍膽石斑魚種建立親緣關係鑑定技術，以利各養殖場內進行親緣管理，使近親交配的機率減至最小。本研究同時採用微衛星及粒線體二種基因系統，作為判別龍膽石斑種內族群親緣遠近之檢驗模式。於粒線體系統當中，選用不受演化壓力之高變異控制區序列片段，作為同一交配世代之親緣辨認工具；本研究共完成共3個養殖場次、118尾龍膽魚鰭樣本之控制區序列分析，依其核苷酸點變異分化出42種單倍基因型，並計算各基因型間之遺傳距離，以此作為龍膽種魚場內同世代分池交配依據。此外，本研究另採微衛星系統作為親代與子代之親緣辨認工具，以阻斷種魚場內上下二代近親交配之可能性，本研究共篩選出6組微衛星基因座，符合哈溫平衡定律，且為非連鎖之獨立基因座，彼此間之多樣性對偶基因組合亦擁有極高之排除親緣誤判機率，可作為龍膽種魚場內親子鑑定工具。由本研究結果證實，當粒線體與微衛星遺傳資訊系統結合，將可成為有效的台灣養殖龍膽石斑親緣管理方法。

Giant grouper is an economically important fish species in Taiwan’s aquaculture industry. Thus, we are attempting to develop a technique that can investigate the parent-offspring/sibling relationship of giant grouper. This technique will improve the inbreeding management of fish farms and minimizes the chance of close relative mating to as lowest as possible. In this study, we would like to apply two systems which are microsatellite and mitochondrial genetic markers as tools to analyze genetic distance of giant grouper population. The high variable mitochondrial D-loop sequence is selected to study the siblings’ relationship of giant groupers. Analysis of data collected from 3 fish farms, with total samples of 118 giant groupers, we found and categorized 42 haplotypes as well as calculate their genetic distance. Genetically close relative giant groupers will be separated to different farms. Moreover, in this project, microsatellites system was applied as parent-offspring investigation tool. 6 different microsatellite loci were used as marker, which are obeying Hardy-Weinberg equilibrium and do not exhibit genetic linkage within each other. Therefore, with high number of possible combinations could avoid chance of misinterpretation and can be practically in aquaculture industry. Combining both techniques would enhance the effectiveness in Taiwan’s giant grouper inbreeding management.

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