本研究主要的貢獻為，建構出新型的平面式細胞共同培養裝置，並且將其應用於有施予、以及不施予流體剪應力的不同培養環境變異下，以探討內皮細胞與平滑肌細胞之互相影響與遷移狀態。此方法是利用毛細作用原理，將細胞吸入細胞共同培養裝置中，在特定的反應區內進行精確的分離培養。有別於傳統方式，此平面式細胞共同培養方法是以創新技術將兩種不同的細胞進行分離，以微米的間距培養於同一晶片上，並且可同時觀察細胞間的交互誘導作用與遷移情況。本研究亦使用CFDRCTM套裝軟體，模擬出循環式流體裝置中的流場分佈方向，以及可施予細胞的流體剪應力大小，並用實驗佐證其結果。

主要研究成果為成功的發展出對兩種不同細胞進行分離且共同培養技術，亦製作出低成本且多功能的微壓印顯微系統與循環式流體裝置，並運用於細胞定位以及探討剪力流對細胞影響之實驗。在剪力流對正常細胞與癌化細胞之黏附性的比較結果部分，正常細胞可承受的流體剪應力是癌細胞的2.3倍以上。在剪力流對細胞成長方向性的改變之結果部分，心肌細胞在承受5.11 dyne/cm2的流體剪應力時，會朝著流場方向排列成長；內皮細胞在承受8.12 dyne/cm2的流體剪應力時，會朝著流場方向排列成長。在施予與未施予流體剪應力的不同環境下，分別探討兩種不同細胞以500 um間距分離且共同培養於同一晶片上之結果部分，（1）內皮細胞與平滑肌細胞互相接觸的速度，在沒有流體剪應力的作用下，會比在有流體剪應力的作用下還要快，故流體剪應力可以達到干擾細胞互相接觸的效果、（2）流體剪應力只會抑制平滑肌細胞的遷移速度，但對內皮細胞則影響較小、（3）在內皮細胞與平滑肌細胞共同培養時，並無互相誘導作用，其原因是500 um間距仍然過大，導致兩種細胞的影響變小。

The contribution of this study is that a novel cell co-culture device was established that can be utilized under environment of shear stress. The method utilizes the capillary theorem to orientate cells in specific area and manages to separate and co-culture two different types of cell between micrometer distance for observation of the interaction and cell transmigration, comparing to the traditional co-culture methods. In this study, the fluid field and shear stress about recyclable fluidic device reaction area are simulated using CFDRCTM and later experiments are carried out to testify the simulation.

Our primary achievement in this study is to have successfully developed the cell co-culture method and fabricated microscopy-based microcontact printing and recyclable fluidic device for low-cost and high performance. The flow shear stress that normal cell can yield is at least 2.3 times of that of cancer cell, comparing normal cell adhesion with cancer cell adhesion under flow shear stress. The results of cell growth direction under flow shear stress show that yielding 5.11 dyne/cm2 of shear stress, H9c2 cells will grow along flow direction while yielding 8.12 dyne/cm2 of shear stress, endothelial cells grow along flow direction. Placing under flow shear stress or non-flow shear stress, we observed and studied the different results of separating endothelial cells and smooth muscle cells in 500 um space and co-culturing them, (1) the touch velocity of endothelial cells and smooth muscle cell under non-flow shear stress is bigger than that under flow shear stress, indicating shear stress can suppress the touch velocity of endothelial cells and smooth muscle cell, (2) though the flow shear stress can suppress the touch velocity of smooth muscle cell, it has a small effect on endothelial cells, (3) there is no induced reaction between endothelial cells and smooth muscle cells no matter they were co-cultured under flow shear stress or not, due to the large distance of 500 um makes the effect of endothelial cells and smooth muscle cells become small.

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