本論文中開發了一種新的電動微流體裝置，該裝置由鎢絲所製成的尖銳導電針尖組成，利用尖端周圍的強電場和電荷極化效應，能夠產生強大的交流電動流動和膠體及分子捕獲效應。此外，觀察到從尖端發出因交流電所致之噴流，此噴流與經典的Landau-Squire噴流明顯不同。該裝置還可以非常有效地捕獲奈米粒子量子點（QD），這促進了螢光共振能量轉移（FRET）分子檢測。
在第三章中，本文提供了有關如何設計適當的電極設置，然後在去離子水中觀察不同交流電頻率下粒子及分子的流動和捕捉現象。首先使用微米級的膠體粒子，在高頻1MHz下，本文發現到尖端噴流，此噴流成因為尖端附近焦耳熱強導致局部的交流電熱流(ACET)，在低頻1KHz下，本文觀察到因歐姆充電機制的交流電滲流所產生的漩渦，此漩渦可以將粒子掃至針尖，有助於尖端附近的正介電泳(p-DEP)捕捉，這種交流電滲流(ACEO)輔助正介電泳(p-DEP)捕捉的方法也可以用於奈米粒子QD上，可以將尖端周圍的QD捕捉到探針上，當本文試圖捕捉λDNA大分子時，發現在5KHz時觀察到明顯的噴流，這次的噴流並非是交流電熱(ACET)所引起，而是由尖端附近的法拉第充電機制的交流電滲流所造成，並且還伴隨著逆時針方向的歐姆充電機制的交流電滲流漩渦。
在第四章中，因於第三章觀察到QD可被有效的捕捉，本文使用這些被捕獲的QD作為FRET供體，並在交流電場下對單鏈DNA（ssDNA）進行FRET。本文發現在電場開啟後可以立即檢測到明顯的FRET訊號。本文進一步使用λDNA作為骨幹，使其與QD結合。
在第五章中，為了使第三章中觀察到的交流電熱噴流現象更加明顯，本文用高電導度的DNA緩衝溶液來代替低電導度的去離子水。本文發現噴流可以變得非常明顯，且於探針後端粒子會匯集於探針上，在尖端處粒子會從針尖遠離尖端。
在第六章中，本文討論了交流電熱(ACET)和交流電滲流(ACEO)噴流現象背後的機制，並根據電壓、圓錐角、流體性質和頻率推導出相對應的尺度。這些特徵與經典的Landau-Squire噴流的特徵截然不同。
鑑於交流電所生成的強大流動和捕獲現象，該裝置可用於快速且提升檢測訊號以及促進分子結合。因此，它可能具有加速晶片分子檢測的用途。

In this thesis, We develop a new electrokinetic microfluidic device using a sharp conducting tip made of a tungsten conical needle. Utilizing strong electric field and charge polarization effects around the tip, this device is capable of generating intensified AC electrokinetic flows and colloidal/molecular trapping effects. In addition, for the first time, an AC jet emanating from the tip is observed, markedly different from the classical Landau-Squire jet. This device further allows me to trap quantum dot (QD) nanoparticles very efficiently, providing me an advantage of facilitating molecular detections using Fluorescence Resonance Energy Transfer (FRET).

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