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研究生： 林冠辰
Lin, Guan-Chen
論文名稱： 嵌入式快速檢測系統開發應用於人類絨毛膜促性腺激素之研究
Development of Embedded Rapid Test System for Human Chorionic Gonadotropin Strip
指導教授： 林裕城
Lin, Yu-Cheng
學位類別： 碩士
Master
系所名稱： 工學院 - 工程科學系
Department of Engineering Science
論文出版年： 2015
畢業學年度： 103
語文別： 中文
論文頁數： 94
中文關鍵詞： 呈色檢測 、快速響應矩陣碼 、人類絨毛膜促性腺激素 、影像處理 、嵌入式
外文關鍵詞： rapid test system, QR code, human chorionic gonadotropin, image processing, embedded
相關次數： 點閱：77  下載：3 
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    • 本研究成功開發出具有高線性度、高靈敏度及高穩定度之嵌入式快速檢測系統，利用免疫反應的呈色結果作為檢測標的，使免疫呈色反應之偵測在定量分析上更加精確。本研究開發之嵌入式快速檢測系統包含硬體及軟體兩部分，在硬體方面設計出一台呈色判讀儀主體，裝載Advanced RISC Machine (ARM)開發板、Universal Serial Bus (USB)攝影機、LED光源、LCD點陣式液晶顯示器、LED燈號顯示、整合式電路板、極限開關及光學暗房，並製作出兩種可替換式載台分別放置檢測卡匣及快速響應矩陣碼。在軟體部分使用Python整合模組Zbar、OpenCV、NumPy及Matplotlib撰寫出影像處理程式。由實驗結果可以發現本研究所開發之嵌入式快速檢測系統在對人類絨毛膜促性腺激素檢測試劑量測下，可以在無人工調整情況，正確讀取Control、Test線(C、T線)位置，並建立出線性度極佳之濃度與灰階值校正曲線，作為量化分析的依據，該曲線決定係數可達0.98，在人類絨毛膜促性腺激素濃度極限偵測下，可量測最低濃度至6.25 mIU/mL，成功地突破傳統閾值25 mIU/ mL的判定，在重複實驗下仍可保持良好的精準度(相對標準差<10 %)。

    This thesis presents an embedded rapid test system with high sensitivity and stability for human chorionic gonadotropin. The inspection is based on immunochromatographic assay which is widely applied to human chorionic gonadotropin detection. In combination with the immunochromatographic strip, the embedded rapid test system is able to detect and calculate the concentration of the sample. The system is composed of hardware and software parts, and the layout of rapid reader was designed by SolidWorks and AutoCAD. The analyzing programs including a main program, QR code and image processing were compiled by MATLAB. After verification of each parts of the system, we found that the rapid test system successfully detect the minimum 6.25 mIU/mL of human chorionic gonadotropin reagent.

    摘要 I Extended Abstract II 誌謝 VI 目錄 VII 圖目錄 XII 表目錄 XVI 第一章 緒論 1 1-1 研究背景 1 1-2 免疫分析法 2 1-2-1 免疫分析基本理論 3 1-2-2 抗原與抗體定義 3 1-2-3 抗原與抗體結合力 5 1-2-4 免疫分析檢測種類 6 1-2-5 免疫分析偵測方法 7 1-3 免疫反應試紙 10 1-4 人類絨毛膜促性腺激素簡介 12 1-5文獻回顧 13 1-5-1人類絨毛膜促性腺激素之檢測 13 1-5-2影像判讀簡介 15 1-5-3影像判讀之發展 17 1-6 研究動機與目的 21 1-7章節架構 22 第二章 嵌入式快速檢測系統之系統設計與製作 24 2-1 嵌入式快速檢測系統之系統流程 24 2-1-1 資訊匯入：QR code 27 2-1-2 影像處理之流程 28 2-1-3 讀取C、T線之演算法：半波峰 32 2-2 呈色判讀儀設計 34 2-2-1 呈色判讀儀外殼設計 34 2-2-2 整合式電路板設計 35 2-2-3 呈色判讀儀結構設計 36 2-3 呈色判讀儀製作 38 2-3-1 整合式電路板 38 2-3-2 呈色判讀儀 39 2-4 程式開發環境建立 40 2-4-1 開發板選用：ARM_Raspberry pi 40 2-4-2 作業系統選用：Raspbian 42 2-4-3 程式語言選用：Python 44 第三章 實驗與研究方法 46 3-1 實驗儀器 46 3-1-1 呈色判讀儀硬體架構 46 3-1-2 感光元件 47 3-1-3 LED光源 47 3-1-4 極限開關 48 3-2 實驗藥品 49 3-3 設備及軟體測試 50 3-3-1 QR code測試 51 3-3-1-1 QR code產出與測試 51 3-3-1-2 QR code形變測試 52 3-3-2 極限開關測試 52 3-3-3 C、T線判讀測試 53 3-3-3-1 C、T線讀值位置測試 53 3-3-3-2 C、T線讀值範圍測試 54 3-3-3-3 灰階線性度測試 54 3-3-3-4 色階線性度測試 56 3-3-3-5 色階靈敏度測試 57 3-3-3-6 色階偵測極限 58 3-4 嵌入式快速檢測系統整合測試 58 3-4-1市售hCG試紙測試 58 3-4-2 C、T線之正確判讀位置驗證 59 3-4-3 導入擬合曲線之濃度測試 59 第四章 結果與討論 60 4-1 嵌入式快速檢測系統之實現 60 4-2 QR code測試結果 62 4-2-1 產出QR code及測試結果 62 4-2-2 QR code形變測試結果 63 4-3 極限開關測試結果 64 4-4 C、T線判讀測試結果 65 4-4-1 C、T線讀值位置測試結果 65 4-4-2 C、T線讀值範圍測試結果 68 4-4-3 灰階線性度測試結果 71 4-4-4 色階線性度測試結果 74 4-4-5 色階靈敏度測試結果 76 4-4-6 色階偵測極限 79 4-5 嵌入式快速檢測系統整合測試結果 80 4-5-1 hCG試紙測試結果 80 4-5-2 C、T線之正確判讀位置驗證結果 84 4-5-3 導入擬合曲線之濃度測試結果 85 第五章 結論與建議 88 5-1 結論 88 5-2 建議 90 參考文獻 91

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