本研究主要針對甲苯共代謝三氯乙烯現地生物復育之生物阻塞工程瓶頸，藉由管柱濾料浮動化方式之連續流管柱試驗，探討管柱中生物阻塞現象之生成機制及影響因子，實驗共分為兩階段，第一階段為生物阻塞試驗，第二階段為濾床浮動化試驗。
　　第一階段試驗中，現地混合菌確實適合使用於甲苯共代謝三氯乙烯，在管柱操作第5天後，濾床即開始出現阻塞情形，水力傳導度由啟動時之60 cm/min，降至結束時之32 cm/min，且管柱中進流端濾料顏色亦隨操作天數增加而由白轉黃褐色。
　　第二階段試驗中，甲苯及三氯乙烯之初始濃度分別為20 mg/L及1 mg/L，初始菌數約為5.5×104 CFU/mL，操作時間均為15天，水力傳導度隨濾床濾料膨脹率增加而有所提升，當膨脹率為10%、20%、40%時，水力傳導度分別為100 cm/min、150 cm/min、260 cm/min，此時甲苯去除率為95%、75%、60%及三氯乙烯去除率為65%、45%、20%，當膨脹率越高時，因水流剪力及濾料顆粒碰撞摩擦導致微生物不易生長，而造成甲苯及三氯乙烯去除率降低。綜合研究結果，以膨脹率10%具有明顯之生物阻塞改善作用，且能達到一定程度之三氯乙烯去除率。

　　The study focused on the bioclogging problem of in-situ cometabolic remediation of trichoroethylene by toluene injection. To discuss the mechanism and effect factors of the appearance of bioclogging in column by using floating filter of continuous column test. The experiment divided into two parts, the first stage was bioclogging test, and the second stage was floating filter test.
　　In the first stage, in-situ mixed strains were suitable to make use of the cometabolic degradation of trichloroethylene by toluene. After the fifth day, the appearance of clogging was observed in the filter bed. In the test period, the hydraulic conductivity reduced from 60 cm/min to 32 cm/min. Also, the color of supporting media at the inlet was from white to brown when the operating days increased.
　　In the second stage, the initial concentration of toluene and trichloroethylene were 20 mg/L and 1 mg/L. The initial colonies of toluene cometablic bacterium were 5.5×104 CFU/mL, and the operating days all were 15 days. The hydraulic conductivity increased when the dilation rate of supporting media increased. When the dilation rate were 10%、20%、40%, the hydraulic conductivities were 100 cm/min、150 cm/min、260 cm/min, respectively. The toluene removal efficiency were 95%、75%、60%, and the trichloroethylene removal efficiency were 65%、45%、20%, respectively. When the dilation rate was higher, microorganism grew difficultly because of the shear force and the friction from supporting media. It made the toluene and trichloroethylene removal efficiency reduce. In conclusion, as the dilation rate was 10%, it had obvious improved the appearance of bioclogging, and could obtain a better trichloroethylene removal efficiency.

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