本研究成功開發出高穩定度之桌上型快速體外檢測試劑免疫分析儀，利用電腦數值控制(Computer numerical control，CNC)雕刻機製作出分析儀檢測設備，並找出低成本且穩定的結構環境。光學檢測部分搭配卡榫結構的定位系統，將檢測卡匣定位於檢測範圍內，接著以免疫反應呈色結果作為檢測標的，使免疫呈色反應偵測於定量分析上更加精確。研究開發之桌上型檢測環境架構包含外殼開發、硬體設備及軟體驗證三個部分；桌上型快速檢測系統外殼開發部分設計出一台分析儀主體及光學暗箱，並製作出多功能載台，正反面分別為檢測卡匣放置區與快速響應矩陣碼黏貼區；硬體設備方面，裝載Raspberry Pi zero w開發板、Universal Serial Bus(USB)攝影機、LED光源、LCD觸控式液晶顯示器、RTC模組(Real-time clock)、導光板、反射片；軟體驗證部分使用Python整合模組Zbar、OpenCV、NumPy及Matplotlib的影像處理程式。由研究結果可以發現，桌上型快速體外檢測試劑免疫分析儀能夠精準達到將檢測卡匣定位於檢測範圍內之目的，並在滴入糞便潛血檢測試劑下，能正確建立出線性度濃度曲線，作為量化分析依據，該曲線R^2值大於0.99，並且在重複實驗下能可保持良好的穩定度(變異係數CV<5%)。

This thesis presents a highly stable desktop rapid test system for vitro diagnostic. The inspection is based on immunochromatographic assay, which is widely applied to fecal occult blood (FOB) detection. Combined with the immunochromatographic strip, the desktop rapid test system is able to detect and calculate the concentration of sample. The system is composed of hardware and software parts, and the layout of rapid reader is designed by SolidWorks. The analyzer was produced by computerized numerical control(CNC) engraving machine. For the optical detection, we use the buckle structure as the detection and positioning device.

[1]衛生福利部統計處，2017，https://goo.gl/nHqpKM
[2]糞便檢查，國立陽明大學附設醫院，2013，https://goo.gl/kuLAZ3
[3]朱益民，天主教耕莘醫療財團法人，2012，https://goo.gl/8U4hHb
[4]衛生福利部，台北醫院，https://goo.gl/JV4qe1
[5]人體直腸與結腸，香港中文大學賽馬會大腸癌教育中心
[6]R. Bellisario, R. B. Carlsen and O. P. Bahl, “Human Chorionic Gonadotropin Linear Amino Acid Sequence of The α Subunit,” Journal of Biological Chemistry, 248, pp. 6796-6809, 1973.
[7]R. B. Carlsen, O. P. Bahl and N. Swawinathan, “Human Chorionic Gonadotropin Linear Amino Acid Sequence of The β Subunit,” Journal of Biological Chemistry, 248, pp. 6810-6827, 1973.
[8]R. H. Garrett and C. M. Grisham, Biochemistry, Saunders College Publishing, 1995.
[9]廖國棠，金奈米粒子標記物在免疫分析、DNA 序列分析及微管道晶片系統分析上的應用，國立中山大學化學研究所博士論文，民國九十四年。
[10]E. Engvall and P. Perlmann, “Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes,” Journal of Immunology, 109, pp. 129-135, 1972.
[11]T. Watanabe, Y. Ohkuno, H. Matsuoka, H. Kimura, Y. Sakai, Y. Ohkaru, T. Tanaka, and Y. Kitaura, “Development of a simple whole blood panel test for detection of human heart –type fatty acid-binding protein,” Clinical Biochemistry, 34, pp. 257-263, 2001.
[12]M. Hedenfalk, P. Adlercreutz, and B. Mattiasson, “Modulation of the measuring range of a radioimmunoassay using an organic water two phase system,” Analytica Chimica Acta, 341, pp. 269-274, 1997.
[13]F. Hardy, L. Djavadi-Ohaniance and M. E. Goldberg, “Measurement of antibody/antigen association rate constants in solution by a method based on the enzyme-linked immunosorbent assay,” Journal of immunological methods, 200, pp. 155-159, 1997.
[14]P. Onnerfjord, S. Eremin, J. Emneus and G. Marko-Varga, “Fluorescence polarisation for immunoreagent characterization,” Journal of immunological methods, 213, pp. 31-39, 1998.
[15]J. A. Schmid and A. Billich, “Simple method for high sensitivity chemiluminescence ELISA using conventional laboratory equipment,” BioTechniques, 22, pp. 278, 1997.
[16]C. A. Janeway, P. Travers, M. Walport, and M. J. Shlomchik, Immunobiology, 5th edition, Garland Science, 2001.
[17]J. Lapthorn, D. C. Harris, A. Littlejohn, J. W. Lustbader, R. E. Canfield, K. J. Machin, F. J. Morgan and N. W. Isaacs, “Crystal structure of human chorionic gonadotropin,” Nature, 369, pp. 455-461, 1994.
[18]J. G. Pierce and T. F. Parsons, “Glycoprotein Hormones: Structure and Function,” Annual Reviews Biochemistry, 50, pp. 465-495, 1981.
[19]莊書蓁，產品造形與工程設計對應模式之研究，碩士論文，國立雲林科技大學工業設計研究所，2003
[20]鄭旭成，塑膠外殼卡扣接合結構之結合力分析與最佳化設計，碩士論文，國立台北科技大學機電整合研究所，2009
[21]楊之青，行動電話外殼卡扣之CAE分析，碩士論文，台灣科技大學機械工程研究所，2006
[22]嚴瑞雄，台灣工具機產業發展概述，中工高雄會刊，第18卷，第4期
[23]柯智虔，犬關節膝蓋骨脫臼防止植體與其手術工具之設計製造及分析，碩士論文，中興大學機械工程研究所，2008
[24]Journal of Science and Engineering Technology, Vol. 8, No. 1, pp. 1-13 (2012)
[25]英貴爾(INNGRIT)有限公司，September 2, 2018, from Aol Tech on the World Wide Web: https://goo.gl/JJC54o
[26]田韶鵬，黃正東，吴森，曹正策，多工位共用安裝的夾具定位布局
優化設計，武漢理工大學學報，第27卷，第8期，2005
[27]S. Birken, Y. Maydelman, M. A. Gawinowicz, A. Pound, Y. Liu and A. S. Hartree, “Isolation and characterization of human pituitary chorionic gonadotropin,” Endocrinology, 137, pp. 1402-1411, 1996.
[28]人絨毛膜促性腺激素
http://en.wikipedia.org/wiki/Human_chorionic_gonadotropin.
[29]徐敘瑢, 陳憲偉, 光電材料與顯示技術, 五南文化事業出版, 2007.
[30]R. W. McNaught and J. T. France, “Studies of the biochemical basis of steroid sulphatase deficiency: Preliminary evidence suggesting a defect in membrane-enzyme structure,” Journal of Steroid Biochemistry, 13, pp. 363-373, 1980.
[31]G. Harrison, P. Haffey and E. E. Golub, “A nanogram-level colloidal gold single reagent quantitative protein assay,” Analytical Biochemistry, 380, pp. 1-4, 2008.
[32]W. N. Zhang and Z. Y. Zhao, “Three-color Data Reproduction Algorithm and Implementation for the Color Image Based on CCD and DSPs,” Computer Engineering, 28, pp. 111-113, 2002.
[33]R. Z. Zhou, J. He and Z. L. Hong, “Adaptive Algorithm of Auto White Balance for Digital camera,” Journal of Computer Aided Design & Computer Graphics, 17, pp. 529-533, 2005.
[34]D. Z. Zheng, Z. L. Wu and S. Wang, “Detecting Method of Quantitative Colloidal Gold Test Strip Concentration Based on the DSP Image Processing,” Proc. IEEE 4th International iCBBE Conference (IEEE iCBBE 2010), pp. 1-4, 2010.
[35]H. Y. Jiang and M. Du, “Research on the Detection of Gold Immunochromatographic Assay by the Image Histogram Feature Vectors and Fuzzy C-means,” Proc. IEEE 8th International FSKD Conference (IEEE FSKD 2011), pp. 467-471, 2011.
[36]Richard Lawler, “Raspberry Pi's latest computer costs just $5” Retrieved September 2, 2018, from Aol Tech on the World Wide Web: https://goo.gl/Dp6Ksd