本研究選擇之實驗對象分別為機車隧道、汽車隧道、柴車停車場、都會區及三種拜香燃煙，七種不同環境空氣中之的氣相及固相半揮發性污染物(SVOCs)，使用高流量採樣器PS-1進行採樣，並以Mutatox做基因毒性測試。針對各排放源多環芳香烴化合物以Mutatox進行以了解各排放源的基因毒性，Mutatox基因毒性試驗分成直接(不添加S-9)及間接(添加S-9) 毒性試驗。
於Mutatox試驗中發現，汽車隧道及機車隧道所採到的樣品，不管添加S-9與否，都有基因毒性的存在。而柴油停車場空氣中，不管添加S-9與否，都發現為疑似基因毒性。至於都會區，不管添加S-9與否，都沒有基因毒性的存在。
拜香燃燒方面：在所有拜香燃燒的固相萃取物SOF，在添加S-9後，發現有基因毒性的存在；氣相萃取物XOC方面，在添加S-9後，發現所有拜香燃燒XOC皆為疑似基因毒性。
所有的採樣樣品的固相萃取物SOF比氣相萃取物XOC基因毒性有較高的基因毒性。
本研究利用快速及敏感性佳的Mutatox試驗系統，作為空氣毒性的篩選試驗(screening test)，未來可加速空氣毒物在致突變性和致癌性測試的推展。

Seven airborne samples with varying degrees of contamination. The airborne samples of both particle phase and gas phase of SVOCs were collected using high-volume samplers (PS-1) to asses the genotoxicity of airbone SVOCs. Air samples at seven sites / environments, including a motorcycle tunnel, a gasoline engine tunnel, and a diesel bus parking lot, an urban site in Tainan, plus three incense sticks, were collelcted for genotoxicity assessment using Mutatox test both with and without activation with S-9.
Ambient air at traffic sites, i.e. the gasoline engine tunnel and the motorcycle vehicle tunnel were found to have positive genotoxicity with or without S-9 activation. Extracts from diesel parking lot were found to have only suspected genotoxicity with or without S9 activation. Finally, the urban site was found to be negative genotoxic with or without S-9 activation.
As to the smoke from incense burning, all the SOF of tested incense were found to be positive genotoxic after S-9 activation. The XOC samples of tested incense were found to have suspect genotoxic after S-9 activation. The genotoxicity of particle-associated (SOF) samples was generally higher than those in the vapor phase (XOC) extacts in all tested air pollutions.
This study shows Mutatox assay is an excellent assessment tool for monitoring toxicity of air samples. This in vitro bioassay is fast, convenient, and cost-effective, and can be widely used as for preliminary toxicity screening of environmental samples.

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