慢性腎臟病 (Chronic Kidney Disease, CKD) 的初期並無明顯症狀，因此許多患者因無病識感而忽略其嚴重性，最終導致病情惡化。慢性腎衰竭之併發症中，以高血鉀之立即性風險最大，因為它會造成病患猝死，若能縮短檢測時間及早發現，適時調整飲食，就能保障病患健康、提升醫療便利性。
在本研究中，開發微流體紙基系統包括檢測盒以及血液分離裝置，應用於鉀離子的濃度檢測。利用金奈米粒子結合對鉀離子有較佳選擇性的適體感測器之比色方法，在紙基晶片的反應區注入試劑，並且由RGB推導出鉀離子濃度。所提出的方法有效使用於鉀離子樣品檢測，濃度區間為1.5mM~8mM。實驗結果顯示，使用所開發的流體紙基系統檢測平台，鉀離子檢測的線性度R2近似為0.997，並且與國立成功大學醫院內科部腎臟科合作簽署IRB (Institutional Review Board)，所採集真實病患檢體，紙基系統與成大醫院檢測的結果比較，經過回收率計算100個檢體案例平均落在97%。總體上，結果顯示，所提出的系統為血液鉀離子檢測提供了低成本、製程簡單和可靠的方法。

In this study, a paper-based system include detection box and blood separation device for colorimetric detection of K+ based on AuNPs as probes and the G-quadruplex aptamer as the recognition element was developed. The reaction region of the paper-based chip was implanted with different reagents, and Potassium ions concentration was deduced from RGB-light intensity. The validity of the proposed method was demonstrated using for the detection of potassium ions samples with a concentration range of 1.5-8 mM. The experimental results indicate that linearity expression R2 approximate 0.997 for K+ detection using the proposed paper-based system platform.
In addition, IRB (Institutional Review Board) was signed with the National Cheng Kung University Hospital. The real patient samples were collected. The results of the paper-based system and the hospital were compared, the recovery rate average of 100 sample cases was 97%. Overall, the results show that the Paper-based system in this study have low cost, a straightforward fabrication process, and reliable approach for blood Potassium ions detection.

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