本研究成功地整合分子模版(Molecular Imprinting Polymers, MIP)於一創新之微流體生物晶片，並且運用表面電漿共振(Surface Plasmon Resonance, SPR)生物感測技術檢測生物檢體。當微量生物檢體進樣後，經由微流體晶片中的“蜘蛛網型蠕動式微氣動幫浦”與微氣動閥門驅動至高靈敏度及高專一性之區域性分子模版(Localized MIP)反應區域，並且同時運用表面電漿共振生物感測儀器進行即時性分析。

　　本研究在微流體生物晶片製作方面，利用微機電製程技術將為流體元件及微型溫度控制元件製作於玻璃(BK-7)與聚二甲基矽氧烷(Polydimethylsiloxane, PDMS)等基材之上。其中，流速感測器能校正微氣動幫浦流率之誤差，以達到精確地控制檢體進樣之流率；微型溫度控制晶片精準地控制檢測環境溫度之穩定，使其能精準地使環境溫度維持在37℃，且誤差範圍可控制在0.1℃以內，藉此降低背景雜訊並提高分析訊號之噪訊比。

　　最後，本研究成功地在1×3分子模版/表面電漿共振整合型生物晶片上，同時針對三種不同分子模版(黃體激素、睪酮、膽固醇)進行生物分子吸附檢測，更結合表面電漿共振生物感測器的高靈敏度、高解析度、動態分析及不須事先標定生物分子之特性，使得本研究能偵測到達人體生理濃度之極限。相信此成果可對未來分子模版及生醫疾病檢測分析提供了一種便利且穩定之研究工具。

　　This study reports a novel microfluidic system integrated with localized molecular imprinting polymers (MIP) for surface plasmon resonance (SPR) detection of specific label-free bio-samples. The innovative microfluidic biochip is capable of transporting a specific amount of bio-samples inside multiple microchannels using micro pneumatic micropumps and micro pneumatic valves to sensing regions where multiple MIP films are locally spin-coated such that highly-sensitive, highly-specific bio-sensing could be achieved.

　　The innovative microfluidic biochip is fabricated by using MEMS (Micro-Electro-Mechanical System) technology on glass (BK-7) and PDMS (Polydimethylsiloxane) substrates. The flow sensor can be certain calibrated the error of pumping rate and realized the accurate sample transfer. In addition, since SPR detection could be very sensitive to temperature variation, a micromachine-based temperature control module comprised of micro-heaters and a temperature sensor was used to maintain a uniform temperature with a variation less than 0.1℃ during measurement.

　　The microfluidic MIP/SPR biochips have the potential to be widely used for bio-sensing applications. While compared to large-scale SPR techniques, the developed microfluidic system has several advantages, including labeling-free, high sensitivity, capability of quantitative analysis of nano-scale bio-molecules in real-time fashion.

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