本研究旨在探討表面化學性質與微奈米結構對磷酸鈣（CaP）礦化行為的影響。並藉由製備具空間階層性的仿生界面，模擬生理條件下的成核環境。在第一部份，我們結合自上而下(top-down)和自下而上(bottom-up)的製程策略，以可塑性聚合物，polydimethylsiloxane(PDMS)，作為印章(stamp)，在目標基材表面上建立大面積微奈米結構圖案，另外，我們藉由交聯劑將有序排列的第一型膠原蛋白，以共價作用力轉移至目標基材表面上。礦化結果顯示，固定膠原蛋白的基材上所形成的磷酸鈣晶體粒徑大於無膠原蛋白之對照組，顯示膠原蛋白介面可促進晶體成長；此外，具1.4 µm週期線狀結構的樣品，其礦物粒徑亦略大於2 µm 圓形結構，說明表面幾何形貌對晶體形貌亦具調控效應。
在研究的第二部分，我們以三種具不同官能基的矽烷分子製備自組裝單分子層(SAMs），分別為：具疏水性甲基（–CH₃）的十八烷基三氯矽烷(OTS)、帶正電氨基（–NH3+）的N-[3-三甲氧基甲矽烷基丙基]-1,6-己二胺(AHAP3)，以及具有高度親水聚醚類官能基(–OCH3)的2-甲氧基(聚氧乙烯)丙基-三甲氧基矽烷(PEG)。將這些修飾表面浸入磷酸鈣礦化溶液後，結果卻顯示，最疏水的 OTS表面的礦物覆蓋率最高。我們推測此結果可能是極性官能基在水相環境中形成穩定水化層，屏蔽離子吸附，抑制了初始成核的發生。進一步比較具有微奈米線狀結構與平坦表面的矽烷薄膜後發現，具結構者的礦物覆蓋率顯著提升。
綜合而言，我們的研究結果說明表面能、官能基極性與微奈米尺度的結構，可共同調控磷酸鈣的異質行為。此研究對於發展仿生礦化材料、骨修復塗層與組織工程支架的表面設計提供參考價值。

This study aims to investigate the effects of surface chemistry and structures on the mineralization behavior of calcium phosphate (CaP). In the first part, we combined top-down and bottom-up fabrication strategies using polydimethylsiloxane(PDMS) as a flexible stamp to generate large-area micro-nanopatterns on target substrates. In addition, type I collagen was covalently transferred onto the substrate surface through crosslinking to create an ordered organic matrix. Mineralization results showed that CaP crystals formed on collagen-coated substrates were larger than those on unmodified substrates. Moreover, samples with 1.4 µm line-patterned structures yielded slightly larger mineral particles than those with 2 µm circular features. In the second part, three silane thin films with different functional groups were modified on silicon substrates: hydrophobic methyl (–CH₃, OTS), positively charged amino (–NH₃⁺, AHAP3), and highly hydrophilic methoxy-terminated PEG (–OCH₃). To our surprise, the most hydrophobic surface (OTS) exhibited the highest mineral coverage. Furthermore, a comparison between patterned and flat silane-modified surfaces showed that the presence of patterns significantly enhanced mineral coverage. Overall, our results demonstrate that surface chemistry and structure collectively regulate heterogeneous CaP mineralization. This study provides valuable insights for designing surface-functionalized materials for biomimetic mineralization, bone repair coatings, and tissue engineering scaffolds.

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