肌酸酐為肌酸 (creatine) 與磷酸肌酸 (creatine phosphate) 之代謝廢物，為人體血液與尿液中作為評估腎臟功能的重要指標之一，臨床上常使用傑夫呈色法 (Jaffé reaction)，利用苦味酸 (picric acid) 與肌酸酐之呈色反應進行檢測。本研究期望以電化學方法結合傑夫呈色法以達到更快速且便利之定量手段。以3,5-二硝基苯甲酸 (3,5-dinitrobenzoic acid, DNB) 取代苦味酸，透過分光光度計、螢光光度計和紅外線光譜儀分析DNB與肌酸酐之呈色反應，本研究簡稱為類傑夫呈色法 (modified Jaffé reaction)。
本研究擬將DNB固定於以poly(Pyr-P3CA) 高分子膜修飾的工作電極上，poly(Pyr-P3CA) 是以吡咯 (pyrrole, Pyr) 與吡咯-3-羧酸 (pyrrole-3-carboxylic acid, P3CA) 經由電聚合方式形成的共聚膜。文中除了以分光光度計、螢光光度計和紅外線光譜儀分析確認DNB與肌酸酐之反應性，乃是以電化學電位式進行肌酸酐濃度的校正與檢測。此外不同的電極製備參數亦將於本研究中進行探討，同時，亦以本研究開發的肌酸酐感測器對尿液檢體進行感測與比對，以確認開發的感測器在臨床應用的可行性。

Creatinine, a metabolite from creatine and creatine phosphate, is a key indicator for kidney’s function in human’s bloodstream and urine. In clinical, Jaffé reaction is the most common method to detect creatinine, where picric acid can react with creatinine in alkaline condition. In this study, we use 3,5-dinitrobenzoic acid (DNB) instead of picric acid to achieve a more convenient and faster way in creatinine detection. By UV/Vis spectrophotometer, fluorescence spectrometer and FTIR, we can analyze the reaction between DNB and creatinine, named “modified Jaffé reaction”.
In this research, DNB is immobilized onto the working electrode by poly(Pyr-P3CA) film, where pyrrole (Pyr) and pyrrole-3-carboxylic acid (P3CA) are copolymerized by electropolymerization. We not only discuss the reactivity by UV/Vis spectrometer, fluorescence spectrometer and FTIR, but also optimize creatinine calibration and detection by potentiometry. Last but not least, the fabricated creatinine sensor will apply in urine spicemen detection to ensure the feasibility in clinical application.

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