中文摘要
為了評估酸化槽污泥利用廚餘的產氫能力，不同量的廚餘添加至六組不同初始食微比（S0/X0）的血清瓶裡，結果發現初始食微比越高，氫氣產生速率越高，最大氫氣產量也越高，在初始食微比為10.8 g COD/g VSS，可達到比產氫速率為6.25 mL/g VSS-hr。利用掃描式電子顯微鏡觀察酸化槽厭氧污泥之型態，優勢的菌相型態為類似Clostridium屬的長桿菌。利用Terminal Restriction Fragment Length (T-RFLP)分生的方法進行酸化槽微生物族群監測，分析出圖譜是以片段長度230 bps之Clostridium cluster I, cluster II,和250 bps之Clostridium cluster III之產氫菌群為主。利用交互因子實驗方法設計不同因子對於廚餘酸化槽污泥產氫能力的影響，發現利用垂直式的震盪培養及初始pH值調整為5.5對於最大產氫量有顯著的影響。
在本研究亦操作一3L反應槽，醱酵槽開始啟動在HRT 4天，有機負荷為 20 kg COD/m3/day，pH 值控制在5.5±0.1 範圍內，啟動期試程一加入玉米澱粉當作輔助基質，氫氣的產生速率為1 L H2/L/day，沒有甲烷的產生。試程二的操作，移除玉米澱粉輔助基質，改以全量進流廚餘基質，進流廚餘之有機負荷增加為20 kg COD/m3/day。試程二之平均氫氣產生速率為0.8 mL H2 /L/day，甲烷產生速率為16 mL CH4/L/day，產生之氣體以二氧化碳為主。為求得更佳之產氫效率，試程三改用高雄餐廳廚餘當作進料基質，氫氣的產生速率為0.7 L H2/L/day。試程4提高負荷，縮短水力停留時間至2天，氫氣的產生速率為1.5 L H2/L/day。以T-RFLP方法分析不同試程之菌群結構，發現反應槽試程2、3中皆以256bps之Clostridium Cluster Ⅲ及503bps之C. aldrichii, C. cellobioparum ,C. termitidis(Cluster Ⅲ),C. formicoaceticum(Cluster XI)的菌群為主。

ABSTRACT
The biomass was taken from a 3m3 acidogenesis bioreactor, for a batch study of the biochemical potential of hydrogen production. Six different food-microbial (S0/X0) ratios were conducted in batch biodegradation tests. In the first 30 hours, hydrogen was significantly produced with an initial food-microbial ratio as 10.8 g COD/g MLVSS. The maximum hydrogen production rate was 6.25 mL H2/ gVSS-hr. Observed with scanning electronic microscopy, long rod Clostridium-like group were predominant in the sludge. The molecular method, T-RFLP was applied to detect the acidogenesis microbial community. The T-RFLP result indicated that the potential hydrogen producing bacterium group existed in acidogenesis biological system. A 3-liter bench scale hydrogen CSTR with fill-and-draw operation fermentor was also established. In the first 20 days during the acclimation period, corn starch is added as the auxiliary substrate. The kitchen waste loading rate was 10 kg COD/m3/day, and corn starch loading rate was also 10 kg COD/m3/day. The hydrogen producing rate is 1 L H2/L/day. At run 2, the kitchen waste at the full loading, 20 kg COD/m3/day, was fed to the bioreactor. After a 20-day operation, the hydrogen producing rate is 0.8 mL /L/day. And the methane production is 16 mL CH4/L/day. The biogas consists mainly of carbon dioxide. Run 3 was fed with Kaohsiung kitchen waste, and it gives a hydrogen producing rate of 0.7 L H2/L/day. Run 4 that increase loading rate and decrease hydraulic retention time, was found the maximum hydrogen producing rate was1.5 L H2/L/day.

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