台灣農漁養殖土地有限，大部分的經濟動物都採取集中方式飼養，然而高密度之飼養環境將提升疾病散播率，為預防大規模的疾病傳染，抗生素被廣泛利用於現今的農漁養殖，然其進入到生態系統中累積很可能會衍生出抗藥性問題，故其對環境造成的潛在危害近年來已逐漸受到重視。氧四環素(oxytetracycline, OTC)經常以低劑量添加於養蝦飼料中藉以預防或治療細菌感染並提升蝦子存活率，然而未被完全攝取之OTC很可能殘留於蝦池，甚至透過換水程序進入到自然界中，為一潛在的環境汙染源。本研究主旨為探討養蝦產業使用之OTC於不同環境基質(蝦池水、蝦池底泥、底泥孔隙水)、蝦組織(蝦肉、蝦內臟、蝦血)及飼料當中的殘留濃度，並瞭解OTC於蝦養殖環境中的分布情形，此外，已有文獻指出，有機物質對於有機汙染物於水土環境之濃度分配有著顯著的影響，故本研究也對蝦池水及底泥進行有機碳含量測定，探討有機質之存在是否會影響OTC在不同基質間的濃度。研究使用養蝦業者提供之蝦子、飼料及蝦池樣本進行分析，利用液相層析串聯質譜儀(LC-MS/MS)定量OTC濃度，並以總有機碳分析儀(TOC analyzer)檢測有機碳含量。
研究結果發現，OTC可於蝦池水(3.91 ± 2.76 µg/L)、底泥(17.84 ± 12.34 µg/kg)、底泥孔隙水(7.72 ± 3.98 µg/L)、蝦肌肉(18.11 ± 15.37 µg/kg)、蝦內臟(109.58 ± 85.75 µg/kg)、飼料(28.5 ± 36.18 µg/kg)以及蝦血在兩種萃取液分別是(10.16 ± 14.2 µg/L MCI buffer)、(11.4 ± 18.5 µg/L citric acid-Na2HPO4 buffer)，其中以蝦內臟之殘留濃度最高。比較OTC於不同基質間之分布，發現其在環境介質(池水、孔隙水、底泥)及飼料中的濃度並無和蝦體組織(肌肉、內臟、蝦血)中的含量呈現顯著關係，而蝦血中濃度與肌肉及內臟中的OTC殘留相關性亦不大。在有機質分析部分，池水之有機碳含量與底泥OTC濃度呈現略微負相關，即當水體有機質愈多時，底泥中的OTC含量會減少。

Oxytetracycline (OTC), a commonly used antibiotic in shrimp culturing, is usually added in feed for the treatment of bacterial disease. The residual OTC in shrimp farms could be a potential source of environmental pollution. This study was aimed to understand the correlations of OTC concentration in different matrices, including pond environment (pond water, pond sediment and pore water), shrimp tissues (muscle, viscera and hemolymph) and feed. The influence of organic carbon contents on OTC residues in pond water/pore water/sediment samples were also evaluated. The extraction procedures of OTC from pond sediment and shrimp tissues (muscle, viscera and hemolymph) were developed and a liquid chromatography coupled with a tandem mass spectrometry (LC-MS/MS) was used to quantify the OTC concentration. A total organic carbon analyzer (TOC analyzer) was applied to analyze the total organic carbon content.
OTC could be detected in pond water (3.91 ± 2.76 µg/L), pond sediment (17.84 ± 12.34 µg/kg), pore water (7.72 ± 3.98 µg/L) as well as shrimp muscle (18.11 ± 15.37 µg/kg), shrimp viscera (109.58 ± 85.75 µg/kg), shrimp feed (28.5 ± 36.18 µg/kg) and shrimp hemolymph in MCI buffer (10.16 ± 14.2 µg/L), shrimp hemolymph in citric acid-Na2HPO4 buffer (11.4 ± 18.5 µg/L). Comparing the residual OTC in varied matrices, no significant correlation was found among the pond environment (pond water, pond sediment and pore water) and shrimp tissues (muscle, viscera and hemolymph). The organic carbon content of pond water and OTC concentration in sediment, however, showed negative correlation, implying more organic substances existing in liquid phase may result in less OTC accumulation in pond sediment.

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