幹細胞具備分化的潛能，其中胚胎幹細胞、誘導性多功能幹細胞和成體幹細胞，是應用於再生醫學、組織工程、基因療法與細胞療法的主要素材。成體幹細胞可分為間葉幹細胞和造血幹細胞，間葉幹細胞可從多種組織中取得，其中，胎盤為較初期的組織來源，經由先前的實驗結果證明，由胎盤絨毛組織所分離的幹細胞除了擁有間葉幹細胞特有的細胞標誌：CD73、CD90、 CD105，也具有胚胎幹細胞特有的細胞標誌，例如：TRA-1-60、TRA-1-81、OCT3/4、SOX-2，因此推斷從胎盤絨毛組織分離的幹細胞當中，有一群細胞族群具備胚胎幹細胞的特性。從胎盤絨毛組織分離出的幹細胞，利用免疫螢光染色法比較八種胚胎幹細胞之細胞標誌的關聯性，包含SSEA3、SSEA4、CD9、TRA-1-60、TRA-1-81、TDGF、POU5F1、NANOG，推論胎盤富含複雜的細胞族群，隨著胎盤的分化過程，細胞逐漸流失原始的細胞標誌，但仍有一群幹細胞同時帶有多重胚胎幹細胞的細胞標誌，即為類胚胎幹細胞。將類胚胎幹細胞以不同培養機制培養，發現其細胞型態與胚胎幹細胞的群落型態相似，並且證明以透明質酸作為飼養層，相比無飼養層組別，顯著保留原始的細胞標誌。此研究以胎盤取代胚胎作為萃取多能幹細胞的新來源，解決傳統胚胎幹細胞製備所牽涉的道德問題。

SUMMARY
Stem cells have varying degrees’ differentiation potential and present cellular markers which characterized different types of cells. Stem cells divide into embryonic stem cells (ESC), induced pluripotent stem cells (iPSC), and postnatal stem cells, which are the materials applicate on regenerative medicine, tissue engineering, gene therapy, and cell therapy. Postnatal stem cells contain mesenchymal stem cells (MSC) and hematopoietic stem cells (HSC). In previous studies, the stem cells which isolate from placental villi not only contain cellular markers of MSC which include CD73, CD90, and CD105, but also present ES cellular markers, for example, TRA-1-60, TRA-1-81, OCT3/4, and SOX2. Using eight ES cellular markers which include SSEA3, SSEA4, CD9, TRA-1-60, TRA-1-81, TDGF, POU5F1, and NANOG, to prove placental villi contains embryonic-like stem cells (ESC-like) by immunofluorescent staining (IF). To find suitable environment to culture ESC-like, compare cell morphology under different culture system. Proved that utilize hyaluronan (HA) as feeder layer present colony formation. Processing HA culture system, significantly positive express ES cellular markers. This study work for resolve ethical problem and develop valuable source for pluripotent stem cell therapy.
Key words: embryonic stem cell, placenta, cellular marker, pluripotent stem cell

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