本研究為睪酮分子模版(molecularly imprinted polymer, MIP)的分析應用，主要分成三部分。
第一部分是合成對睪酮分子有高吸附性的塊狀模版，探討聚合時不同的單體與溶劑對模版吸附量與選擇性的影響。選擇MAA與HEMA兩種單體分別合成高分子，結果顯示，以MAA為單體的模版有較高的目標物吸附量74.8ppm與較佳選擇性4.5；而以環己烷比甲醇更適合當高選擇性模版的溶劑。
第二部分為合成薄膜狀的分子模版，探討聚合溫度對吸附力的影響，以及模版對於目標物與競爭物的吸附性質。結果顯示，在45℃有最佳聚合溫度，模版對目標物的吸附量透過Scatchard’s plot可計算出內部的可辨識孔洞數0.41μmole /cm2•g與吸附平衡常數Ka = 9.85 ppm-1。
第三部分則是薄膜分子模版的感測能力，吸附過的分子模版製成電極進行交流阻抗測試。實驗結果顯示，阻抗值隨著吸附液濃度增高與模版吸附量增多而減少。合成模版時，模印分子含量0.5g，單體/交聯劑比值為0.33時，有最高感測靈敏度。目標物與干擾物的感測現象相同但有不同的阻抗值。然而非分子模版卻因為孔洞沒有膨潤現象，使得阻抗值趨勢有不同的結果。每組數據均重複四次以上實驗，並取平均值所得之結果。

In this study, the molecularly imprinted polymer (MIP) with testosterone was investigated in three parts.
The first part refered to the synthesis of bulk polymers having a high affinity for testosterone. Various monomers and solvents for polymerization were examined in order to determine their influence on the binding amount and selectivity. Two kinds of monomers, methacrylic acid (MAA) and 2-hydroxyethylmethacrylate (HEMA), were used to prepare bulk MIP. It was found that the polymer synthesized with MAA monomer had higher selectivity (4.5) and adsorption amount (78.4ppm) than that with HEMA monomer; and the cyclohexane is a better porgen for selective MIP than methanol.
In the second part, thin-film imprinted polymer was synthesized and investigated. The polymerization temperature effect and the adsorption properties for template and analog were in discussion. It were that there was a optimum polymerization temperature (45℃) for membrane MIP. By calculating the adsorption amount of the MIP through Scatchard’s plot, equilibrium constant (9.85 ppm-1) and the amount of recognition sites (0.41μmole /cm2•g) could be found.
The last part is about the sensing ability of the membrane MIP. MIP electrodes was produced by the adsorbed thin film MIP and then tested A.C. Impedance spectroscopy. Charge transfer resistance of the adsorbed MIP decreased with the increase of the adsorption amount and adsorption solution concentration. The MIP composed with 0.5g template and 0.33 monomer/cross-linker ratio had the highest sensitivity. Sensing the template and the analog had the same phenomenon but different resistance values. Non-imprinted polymer had opposite electrochemical behavior compared with imprinted polymer because of the swelling effect of the recognition site. All data were got from experiments at least four times.

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