Hz-1病毒基因組已於2002年定序完成，Hz-1病毒的基因組全長有228,108個鹼基對，計有154個ORF及1個不轉譯的基因PAT-1，經NCBI的BlastP比對後，第131個ORF的比對結果是DNA聚合酶，有趣的是其中的DNA聚合酶所比對到最相似物種為一種耐熱古細菌(Aeropyrum pernix)，此種古細菌的最適生存溫度為95°C。
本論文探討Hz-1病毒DNA聚合酶基因表現模式及利用重組病毒技術大量製備Hz-1病毒的DNA聚合酶，並分析酵素活性。
RT-PCR及Northern blot的結果皆顯示，Hz-1病毒的DNA聚合酶從感染後4小時開始轉錄出RNA且一直持續至24小時都可偵測到RNA的表現，因此Hz-1病毒DNA聚合酶屬於早期基因。
首先以Hz-1病毒感染TN368細胞8小時後的RNA進行反轉錄聚合酶連鎖反應，取得Hz-1病毒DNA聚合酶的全長cDNA，全長為3.5 kb，隨後再與傳送載體接合，與線形AcMNPV基因體DNA進行同源重組後轉染至Sf9細胞中，取得重組病毒後，再感染Sf9細胞，最後以His-bind resin純化Hz-1病毒的DNA聚合酶，進行活性分析。
活性分析結果顯示，Hz-1病毒DNA聚合酶於70℃下20分鐘仍有酵素活性。

Genomic sequence of Hz-1 virus has been completely finished in 2002. The Hz-1 virus genome is 228,108 bp containing 154 open reading frames (ORFs) and one untranslated PAT1. The ORF131 is a putative DNA polymerase. Interestingly, the DNA polymerase of Hz-1 virus has a strong similarity to that of Aeropyrum pernix, a strictly aerobic hyperthermophilic crenarchaeon with its optimal growth at 95°C.
DNA polymerase of Hz-1 virus was expressed using recombinant virus and E. coli expression system. Enzymatic activities of the expressed proteins were analyzed. Total RNA was extracted from TN368 cells infected with Hz-1 virus at 8 h post infection (h p.i.). The full-length cDNA of DNA polymerase adding (His)6-tag at both 5’-and 3’-ends were obtained using RT-PCR, and cloned into T-vector. The cDNA fragments was released with Bgl II enzyme and cloned into a transfer vector, pVL1392, and the released products were named as pVL1392-NDPOL and pVL1392-CDPOL for both N’-tag and C’-tag, respectively. These two constructed transfer vectors were mixed well with linear AcMNPV genomic DNA and co-tansfected into Sf9 cells. Recombinant viruses, AcNDPOL and AcCDPOL, were plaque purified and grown to high titers for protein purification. DNA polymerase was purified with His-bind resin and enzymatic activities were analyzed.
Results showed that NDPOL was stable after incubating at 70℃for 20 min, and CDPOL was stable after incubating at 70℃ for 10 min.

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