將陰乾的日本黃柏（Phellodendron japonicum Maxim.）葉部，以甲醇熱迴流萃取、過濾，濾液經減壓濃縮後，再利用氯仿與水進行分配萃取，得到氯仿層及水層。各劃分層利用管柱層析、薄層層析、高效能液相層析及再結晶等方法分離純化。目前共分離得到36個化合物，其中33個化合物經光譜分析，確定其化學結構為：Flavonoids：phellamurin、amurensin、quercetin、(2R)-phellodensin-F、phellodensin-A、phellodensin-B、6'-O-acetyl amurensin、6'-O-acetyl phellamurin等8個化合物；Coumarins：umbelliferone、scopoletin、scoparone、esculetin、columbianetin等5個化合物；Benzenoids：benzoic acid、vanillic acid、methylparaben、methyl p-anisate、p-hydroxybenzoic acid、p-coumaric acid、methyl p-hydroxycinnamate、methyl caffeate、p-hydroxybenzaldehyde、caffeic acid、phellojaponin-A等11個化合物；Triterpenoids：lupenone、3-epilupeol、friedelin、β-sitosterol等4個化合物；Amides：N-p-coumaroyltyramine、N-trans-cinnamoyltyramine等2個化合物；Alkaloids：rutaecarpine、3-formylindole等2個化合物；Ionone：grasshopperketone等1個化合物。其中6'-O-acetyl amurensin、6'-O-acetyl phellamurin、(2R)-phellodensin-F、phellojaponin-A等四個為天然界首次分離得到之新化合物。

　　Four new compounds, 6'-O-acetyl amurensin, 6'-O-acetyl phellamurin, (2R)-phello- densin-F and phellojaponin-A, together with twenty-nine known compounds: Flavonoids: amurensin, phellamurin, quercetin, phellodensin-A, phellodensin-B; Coumarins: umbelliferone, columbianetin, scopoletin, scoparone, esculetin; Benzenoids: benzoic acid, vanillic acid, methylparaben, methyl p-anisate, p-hydroxybenzoic acid, p-coumaric acid, methyl p-hydroxycinnamate, methyl caffeate, p-hydroxybenzaldehyde, caffeic acid; Triterpenoids: lupenone, 3-epilupeol, friedelin, β-sitosterol; Alkaloids: rutaecarpine, 3-formylindole; Amides: N-p-coumaroyltyramine, N-trans-cinnamoyltyramine and Ionone: grasshopperketone were isolated from the leaves of Phellodendron japonicum Maxim. Their structures were determined by 1H-, 13C-, 2D-NMR and MS spectroscopic analyses.

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