本研究提供一個簡單、低汙染之水相製程，透過控制寡聚胺酸組成、四乙氧基矽烷濃度、金屬前驅物濃度與種類，合成出二氧化矽/寡聚胺酸膠體粒子、中孔洞二氧化矽與金屬奈米粒子/二氧化矽複合材料。首先利用波蘿蛋白酶聚合出賴胺酸與酪胺酸共聚物與賴胺酸與苯丙胺酸共聚物之寡聚胺酸，透過控制四乙氧基矽烷濃度進行動態水解縮聚反應，調控合成之二氧化矽/寡聚胺酸膠體粒子之二氧化矽含量，並間接調控粒徑介於150~430奈米，等電點介於3.2~4.2。高溫鍛燒後得到中孔洞二氧化矽，比表面積介於173~630 m2 g-1，孔洞大小介於3~5nm，為寡聚胺酸之β-摺板作為模板形成之孔隙。進一步利用寡聚胺酸上具還原金屬離子能力，成功將金和銀奈米粒子擔載在二氧化矽/寡聚胺酸膠體粒子上，再透過賈凡尼置換反應將低電動勢之銀奈米粒子部分置換成高電動勢之鈀奈米粒子。高溫鍛燒後得到金屬奈米粒子/二氧化矽複合材料，實驗結果顯示金屬奈米粒子具還原對硝基苯酚之觸媒效果。此外，不同金屬前驅物濃度與金屬種類，也會影響金屬奈米粒子形成之還原性、粒徑大小和分散性。

In this study, we report the facile green synthesis of oligopepeptides, silica/peptide colloidal particles, mesoporous silicas and metal/silica hybrid materials. Chemoenzymatic synthesis of peptides is a clean chemistry technique that allows fast prodution under mild conditions. Here, we synthesized oligo(L-lysine-co-L-tyrosine) [oligo(KY)] and oligo(L-lysine-co-L-phenylalanine) [oligo(KF)] by bromelain, as well as characterizing in terms of yield, molecular weight , and composition of the oligopeptides. The silica/peptide colloidal particles can be simply fabricated by silica mineralization of oligo(KY) and oligo(KF). Tuning the tetraethyl orthosilicate (TEOS) concentration caused the size of the silica/peptide colloidal particles between 150 and 430 nm, and isoelectric point (pI) between 3.2 and 4.2. After removing the oligopeptide, mesoporous silicas exhibited pore size between 3 and 5 nm by replication of sheet-like peptides. In addition, the as-prepared silica/peptide colloidal particles served as reducing agents for the nucleation of metal nanoparticles (NPs). Next, the Ag/Pd alloys-based silica hybrid materials were obtained through the galvanic replacement reaction. After calcination, the metal/silica hybrid materials were obtained. The experimental data revealed that the metal/silica hybrid materials showed reliable catalytic activity in reducing 4-nitrophenol (4-NP) with a pseudo-first-order reaction rate.

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