本研究以感應耦合電漿原子發射光譜儀(Inductively Coupled Plasma- Atomic Emission Spectrometry, ICP-AES) 之快速分析、低偵測極限及可同時分析多元素的能力，搭配微波消化，建立測定植物中微量元素(鐵、鋅、硒、銅、錳) 含量的方法。並進而對所建立的方法進行驗證，鑑定因素諸如：準確度(accuracy)、精確度(precision)、偵測極限(limit of detection, LOD)、線性(linearity )與線性相關係數(linear correlation coefficient )等，然後將建立的方法用於保健植物(明日葉、石蓮花、苜蓿芽、牧草、大蒜、香椿) 之分析，同時對這些植物清除DPPH自由基的能力進行探討。

　　本方法，經以美國國家標準與技術研究院(NIST)，之標準參考物質( NIST 1570a、NIST 1573a )予以驗證，其所得結果顯示本方法的準確度、精確度、偵測極限、線性與線性相關係數等，均達預設的標準，具有高度的可信度，能提供未來作為制訂植物中微量元素檢測之標準方法參考。

　　經由本研究分析的結果，保健植物中的微量元素含量如下：明日葉之鐵182.8±1.83 ppm、鋅35.5±2.31 ppm、硒34.9±5.00 ppb、銅10.8±1.17 ppm、錳10.0±0.10 ppm；石蓮花之鐵115.9±4.05 ppm、鋅30.5±0.61 ppm、硒低於偵測極限、銅9.7±0.73 ppm、錳52.1±0.10 ppm；苜蓿芽之鐵93.7±2.06 ppm、鋅58.1±0.52 ppm、硒1966±175 ppb、銅12.6±0.60 ppm、錳18.0±0.40 ppm；牧草之鐵158.2±0.47 ppm、鋅69.4±0.97 ppm、硒63.4±9 ppb、銅11.1±0.52 ppm、錳62.4±0.81 ppm；大蒜之鐵19.7±0.77 ppm、鋅23.5±0.26 ppm、硒1448±181 ppb、銅3.9±0.25 ppm、錳6.1±0.25 ppm；香椿之鐵174.6±4.40 ppm、鋅189.6±5.69 ppm、硒2428±114 ppb、銅28.1±0.51 ppm、錳29.5±0.68 ppm。在抗氧化性方面，經以DPPH法分析得知，各保健植物於200µg/mL時，其清除DPPH自由基的能力分別為：苜蓿芽84.6 %、香椿81.6 %、明日葉75.9 %、牧草56.2 %、大蒜40.8 %、石蓮花為28.7 %。

　　明日葉、石蓮花、苜蓿芽、牧草、大蒜及香椿等保健植物不但含有鐵、鋅、硒、銅和錳等微量元素，在清除DPPH自由基的能力也具有良好的效果，可當作國民膳食之參考依據；若經進一步的加工，可開發成新機能性的食品或素材，及未來應用於預防或治療氧化相關的疾病。

　The purpose of this study is to use ICP-AES, which shows simple, fast, and low detection limitation characteristic, to analyze trace elements after being digested by microwave as well as the quantity of trace elements (Fe, Zn, Se, Cu and Mn) at the same time. This method is to determine and verify for the accuracy, precision, limit of detection, linearity, linear correlation coefficient and it is able to execute the six health plants including, ashitaba, echeveria, alfalfa sprout, alopecoidesr, garlic and toona.
　The method is qualified by testing all of the quality to attribute against the U.S. National Institute for Standards and Technology (NIST) Standard Reference Materials (SRM) of 1570a Spinach leaves and 1573a Tomato leaves. The results were shown the accuracy, precision, limit of detection, linearity and linear correlation coefficient without except requests. The authentic method can be performed by Standard Operate Procedure for trace-elements in the plants.
　Furthermore, this study discovers the abundant of trace elements in the plants and they can scavenge the DPPH radicals. The trace elements have potential to be developed the functional products to prevent the diseases.

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