Ficus microcarpa belongs to Moraceae family is known as banyan or Indian banyan tree and is widely distributed well all over the world. After the banyan tree was planted by the emperor of Japan, banyan tree garden has become one of the famous tourist attractions in Taiwan and landmark of National Cheng Kung University and the spirit of the university stand as well. Therefore, it is no wonder banyan trees are not only major symbol of the university but also representative of Tainan. To regenerate banyan tree, optimizing sterilization process was performed with different concentrations of sodium hypochlorite solution with the different rage of times and it was found that 20 minutes of sterilization time with 1% sodium hypochlorite was optimized for sterilization. To investigate the effects of different incubation condition, three different treatments, 16 hour light and 8 hour darkness photoperiod, a week of darkness followed by continuous photoperiod and continuous darkness, were applied for experiments and it was observed that callus induction can occur under complete dark condition in a low chance but much healthier compared to the other treatments. Different concentrations of plant growth regulators were also added in MS medium and then at least after two weeks of the culture, callus or shoot induction occurred at the same culture. It is possible that a combination of 2 mg/L 6-benzyl aminopurine and 0.1 mg/L naphthalene acetic acid is boundary line between callus and shoot induction.

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