周邊動脈疾病 (Peripheral arterial disease, PAD)是因為周邊動脈血管中斑塊或脂肪堆積造成血流減少，導致血管周邊組織養分供應不足、組織壞死及纖維化。嚴重肢體缺血 (Critical limb ischemia, CLI)是周邊動脈疾病其狀況嚴重的臨床現象。臨床上高齡的糖尿病患者患有周邊動脈疾病的可能性更高。本研究對豬隻誘導糖尿病並建立下肢缺血的手術方法來模擬糖尿病CLI患者。首先使用Alloxan成功誘導豬隻高血糖並維持血糖值在400 mg/dL以上。針對成功誘導糖尿病的豬隻進行下肢的雙側外腸股動脈 (External iliac artery, EIA)血管結紮手術來建立下肢缺血的模型，並以實驗室所研發的BMT gel來治療豬隻右側的EIA結紮處。本研究中使用外觀行為模式評估、電腦斷層掃描、H&E染色、Masson’s trichrome染色及IHC染色來確認藥物療效。本研究顯示，治療後的下肢有明顯的血管新生及組織再生的情況，並且可以觀察到外觀行為模式的改善。我們的研究提供了CLI動物模式治療的良好基礎，提高了後續轉譯到臨床的成功率。

Peripheral arterial disease (PAD) is a decrease in blood flow caused by plaque or fat accumulation in peripheral arteries, resulting in insufficient nutrient supply, tissue necrosis, and fibrosis in peripheral arterial tissues. Critical limb ischemia (CLI) is a severe clinical phenomenon of peripheral arterial disease. In clinical, older diabetes patients have a higher occurrence of peripheral arterial disease. First, induce diabetes in pigs and establish a hyperglycemic pig model to mimic diabetic patients. Alloxan was used to successfully induce hyperglycemia in pigs and maintain blood glucose levels above 400 mg/dL. Next, blood vessel ligation was performed on successfully induced diabetes pigs to induce hind limb ischemia in pigs. In this study, the bilateral external iliac artery (EIA) of the hind limbs in Lan-yu pigs was ligated to establish a model of hind limb ischemia, and the EIA ligation right site of the pigs was treated with BMT gel developed in the laboratory. In this study, behavior patterns, computed tomography, H&E staining, Masson’s trichrome staining, and IHC staining were used to confirm BMT gel efficacy. In this study shows that the hind limbs after treatment have obvious angiogenesis and tissue regeneration, and the improvement of appearance and behavior can be observed. Our research provides a great basis for the treatment of CLI animal models and improves the success rate of subsequent clinical translation.

鄭紫妃，改良之DIB膠於小鼠下肢缺血之治療，國立成功大學生物科技研究所碩士論文，2016。

詹秉鈞，蘭嶼豬下肢缺血動物模式之研究，國立成功大學生物科技與產業科學系研究所碩士論文，2019

靳椏棋，DIW膠對於糖尿病小鼠下肢缺血的作用研究，國立成功大學生物科技與產業科學系碩士論文，2021。

戴偉城，蘭嶼豬之糖尿病誘導對於缺血下肢之影響研究，國立成功大學生物科技與產業科學系碩士論文，2022。

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