眾所周知，糖尿病患者的傷口照護上比一般人還困難許多，因血液中含有高糖份的緣故，造成血液循環不良進而導致患者傷口難以癒合，尤其在患者下肢常常發生組織壞死潰爛的情形，甚至面臨截肢的問題，在臨床上已有非常多案例。因此迫切需要有效的方法來治療照護糖尿病患者的傷口，促進組織再生並預防復發性潰瘍。止血、發炎、增生與成熟期是傷口癒合的四個主要階段，尤其是發炎反應在糖尿病患者傷口中比正常傷口更嚴重。巨噬細胞已被證實在發炎期扮演重要角色，許多研究也指出巨噬細胞異常會影響傷口癒合狀況。因此在本篇研究我們以Alloxan誘導建立糖尿病小豬動物模式，然後在其背部建立大小合適的開放性傷口，並用本實驗團隊具有專利的多孔膠原基質RegDerm進行治療，以說明RegDerm的治療效果和機制。結果顯示，我們的RegDerm敷料與市售產品相比有更好的改善傷口癒合能力；在術後1週觀察到使用RegDerm治療的傷口已從底部快速增生新組織了，且修復後的傷口外觀也較為平整。此外我們還進行組織染色觀察巨噬細胞M1/M2的比率，得出結論RegDerm可能是透過減少M1，增加M2方式，整體有調節發炎與促血管新生的作用。簡而言之， RegDerm的治療效果可能是透過調節修復前期的發炎反應來促使傷口癒合。

Diabetes mellitus (DM) can significantly impair wound healing due to metabolic disturbances. High blood sugar levels cause vascular changes, reducing blood flow and oxygen supply to tissues, which slows down the healing process. The accumulation of advanced glycation end products (AGEs) further disrupts collagen formation and tissue regeneration. Furthermore, diabetes-associated neuropathy may reduce sensation, leading to unnoticed injuries. Poor circulation, immune dysfunction, and delayed cellular repair contribute to prolonged wound healing, making individuals with diabetes more prone to chronic, non-healing wound. In this study, we established the DM pig model by alloxan induction, maintaining at around 200 mg/dL at least one month. Furthermore, we created three wound on pig back which were untreated, treated with different materials, including the competitive product, Integra Dermal, and our Lab material, RegDerm respectively. We evaluated the wound area and wound contraction from measure the appearance, and tissue regeneration in histology. We also study in the changes in macrophage type M1 and M2 during the tissue inflammatory phase. The results showed that the wounds which treated with RegDerm were better than other untreated and Integra Dermal treated group. The RegDerm treatment group's wound area has closed in 14 days after operation. However, we found that regenerated tissue arrangement in RegDerm treatment group were also more similar like normal skin, comparing with other group. In above, the wounds that treated with RegDerm may through regulation of the M1/M2 type during the inflammation. The results indicated that RegDerm not only improving the wound healing but also helping the tissue have better regeneration.

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