本論文試圖發展出一套次飛秒脈衝雷射加工系統搭配稜鏡對與聲光調變器來進行生物組織影像掃描與加工，由於傳統雷射加工系統(即使是奈秒級)，熱效應很嚴重，會有融熔殘渣、微裂縫、精細度差等缺點。而飛秒雷射已可將這些問題做明顯的改善，但一般飛秒雷射的脈衝寬度大都在100 fs左右，這樣的脈衝寬度還是會有熱效應的產生。但是若想要使用脈衝寬度100 fs以下來做加工的話，其色散效應是一個非常嚴重的問題。
研究中使用材料為SF-11的稜鏡對來對整體光路系統進行二階色散的補償，主要補償的元件為聲光調變器與物鏡，補償後之系統脈衝寬度為45 fs，並且利用聲光調變器來當作雷射開關與雷射脈衝選擇器去精確地控制雷射強度的脈衝數量，並對不同大小螢光球和雞腱進行雙光子影像與二倍頻影像掃描，最後也使用不同的脈衝寬度在生物組織上進行加工，我們成功地提高影像之空間解析度以及加工效率，降低熱效應的影響。

In this thesis, we want to develop a sub-femtosecond pulsed laser machining system integrated with prism pair and acousto-optic modulator (AOM) for 3D imaging and processing of bio-tissue. Because of conventional laser machining systems (even at nanosecond pulse) have very serious thermal effect. There are many disadvantages such as melting residues, micro-cracks, and poor fineness etc. They can be significantly improved via using femtosecond laser, but generally speaking, the pulse width of femtosecond laser is about 100 fs, there is still has thermal effect in such pulse width level. Dispersion effect will be very severe issue when machining pulse width is below 100 fs.
The SF-11 prism pair was utilized to compensate overall optical system’s second order dispersion. The major compensation components are AOM and objective. After compensating, the system pulse width is 45 fs, and the AOM was utilized to become laser switch and laser pulse selector for precision control the dose of laser intensity. We get the two-photon image from different sizes of fluorescent beads and second harmonic generation image from chicken tendon. Finally, we also use different pulse width on bio-tissue processing. Improving the image spatial resolution, processing efficiency, and reducing thermal effect have been successfully accomplished.

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