本論文研究主要目的是探討以梔子素(Genipin)、幾丁聚醣(Chitosan)或核醣(Ribose)交聯處理台灣鯛魚鱗膠原蛋白之物理性質與人類腎臟近端小管上皮細胞株(HK-2)生長之探討。在物理性質方面，我們利用掃瞄式電子顯微鏡(SEM)、膨潤度(Swelling ratio)、交聯指數(Ninhydrin assay)、熱變性溫度(Td)進行測試分析。同時也利用人類腎臟近端小管上皮細胞株(HK-2)在膜上進行細胞活性測試。
由結果我們可以發現，利用梔子素(Genipin)、幾丁聚醣(Chitosan)或核醣(Ribose)交聯後的膠原蛋白膜具有好的物理性質以及細胞生長活性。以1%(w/v) chitosan/ 0.10%(w/v)genipin/ 0.06%(w/v)ribose為交聯條件時，有最好的交聯指數(81.71%)；同樣的交聯比例在DSC分析中，也得到高達111.29 ℃的熱變性溫度。然而在HK-2細胞生長率測試實驗中，我們發現梔子素(Genipin)和核醣(Ribose)可以提升細胞生長率，並且發現以1%(w/v) chitosan/ 0.10%(w/v) genipin/ 0.06%(w/v) ribose來交聯膠原蛋白，其細胞生長率比未加入交聯劑的膠原蛋白高出7.8倍。因此，由實驗結果中可得到由台灣鯛膠原蛋白製備成生物材料薄膜最好的組成條件。

This study is to explore the feasibility of preparing the biomaterial from the Taiwan Tilapia scale collagen by cross-linked with genipin, chitosan, and/or ribose. The physical properties measured by scanning electron microscopy (SEM), cross-linked index (Ninhydrin assay), degree of swelling (Swelling ratio), thermal denaturation temperature (Td) were measure. The human kidney proximal tubular epithelial cell line (HK-2) were cultivated on the film to measure the cell growth activity.
It was found that the film prepared by cross-linked with genipin, chitosan, and ribose show better physical properties and cell growth activity. When 1%(w/v) chitosan/ 0.10%(w/v)genipin/ 0.06%(w/v)ribose cross-linked with collagen showed the best cross-link index, 81.71%. And the film prepared with this ratio also showed the highest thermal denaturation temperature, 111.29 ℃. The HK-2 cell growth rate tests showed that ribose and genipin were capable of increasing the growth rate and found when film prepared by cross-linking 1%(w/v) chitosan/ 0.10%(w/v)genipin/ 0.06%(w/v)ribose, the cell growth rate was 7.8 folds higher compared to the collagen alone. Therefore, we have shown the best composition for the Taiwan Tilapia scale collagen to prepare the biomaterial film

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