白點症病毒(White spot syndrome virus，WSSV) 對於蝦類養殖產業造成嚴重的經濟損失，因此了解其致病機制更顯重要。先前實驗室的研究中發現白點症病毒會在感染12小時 (12 hpi) 促使瓦氏效應(Warburg effect)和穀醯胺酸分解作用 (glutaminolysis) ，而這些現象會在感染24小時 (24 hpi) 停止。在本篇研究當中，利用13C代謝流分析 (metabolic flux analysis) 釐清感染白點症病毒蝦血細胞內整體代謝物以及代謝流的改變。在感染白點症病毒12及24小時，白蝦會被注射穩定同位素標定碳追蹤物 (stable isotopic tracer)追蹤，採集蝦血細胞並利用液相層析串聯質譜儀(LC-ESI-MS)分析其代謝物。在感染白點症12小時，利用U-13C6 glucose進行追蹤，發現醣解作用中帶有13C的相關代謝物含量並且其生產速率會提升，而檸檬酸循環中則沒有顯著增加；利用U-13C5 glutamine進行追蹤，發現檸檬酸循環中帶有13C的相關代謝物含量及生產速率都會提升。在感染白點症24小時，利用U-13C6 glucose進行追蹤，發現醣解作用中則沒有顯著增加。從以上得知，在感染白點症12小時，瓦氏效應會驅使醣解作用的代謝流會增加並且促使穀醯胺酸分解作用的代謝流增加，並會利用穀醯胺酸當作葡萄糖外的碳源。

Since the white spot syndrome virus (WSSV) causes serious economic losses, it is important to elucidate its pathogenesis. It has been reported that WSSV induced a Warburg-like effect and glutaminolysis in shrimp at 12 h after infection (hpi), although these phenomena had ceased by 24 hpi. In this study, 13C metabolic flux analysis was used to characterize cellular metabolic states and metabolic fluxes in WSSV-infected hemocytes. At 12 and 24 hpi, white shrimp were treated with uniquely labeled isotopic tracers, hemocytes were collected and liquid chromatography–electrospray ionization-tandem mass spectrometry (LC–ESI-MS) was used to analyze metabolites. At the genome replication stage (12 hpi), using U-13C6 glucose as a tracer, amounts of isotopic labeled glucose-derived intermediates in glycolysis were increased and corresponding production rates were faster than in the control group, although there was no enhancement of the TCA cycle. Using U-13C5 glutamine as a tracer, amounts and production rates of isotopic-labeled intermediates in the TCA cycle were both increased. At the late stage of infection (24 hpi), using U-13C6 glucose as the tracer, enhancement of glycolysis was no longer apparent. Taken together, at the WSSV genome replication stage (12 hpi), high levels of glycolytic intermediates were causing a Warburg effect by triggering glycolysis. In addition, glutaminolysis was also enhanced, providing an alternate carbon source for a glucose-independent TCA cycle.

李春媛，探討穀醯胺酸分解作用於白點症病毒致病機轉之角色，國立成功大學生物科技研究所碩士論文，2015。

蘇美安，雷帕黴素標靶蛋白訊息傳遞路徑在白點症病毒之致病機轉中所扮演的腳色，國立成功大學生物科技研究所碩士論文，2013。

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