胰島素樣生長因數-1 （insulin-like growth factors,簡稱IGF-1）是牛乳的天然成分之一。它是一種多功能的細胞增殖調節因數，同時過量的IGF-1攝入可能會打亂人體的正常的生理機能。近來有研究表明乳牛食用含有激素藥品的飼料會增加牛乳的產量，同時會使牛乳中的IGF-1含量提高。故需建立牛乳中IGF-1的定量檢測方法以確保牛乳的安全性。本文以多反應監測法(Multiple Reaction Monitoring, MRM)為基礎，使用液相質譜對牛乳中的IGF-1進行定量檢測。IGF-1標準品進行胰蛋白酶水解後進行液相質譜檢測，通過Mascot資料庫搜索得到完整的蛋白質生肽圖譜。根據蛋白質體學的原則，總共從5個母離子中選擇了19對MRM離子對用於目標蛋白的定量。從濃度2.77-36.07pg/ul的標準曲線擬合R2值大於0.9。通過單一的MRM離子對，最低定量極限約2.77pg/ul。1-10ng IGF-1標準品被添加進牛乳樣品。標準添加的樣品使用鹽酸乙醇溶液純化，Gn-HCl 及 DTT進行蛋白變性、去雙硫鍵，IAM進行烷基化，胰蛋白酶進行水解，然後進行液相質譜分析。鑒於牛乳中本身的IGF-1含量非常低，通過標準添加曲線，計算得回收率約64%。使用同樣的方法，計算得牛乳中IGF-1的含量約為1.14pg/ul，同時和酶聯免疫吸附測定法（ELISA）獲得的結果進行比較。

IGF-1 is a multifunctional cell proliferation regulation factor and excessive intake of IGF-1 may disturb the body's normal physiological functions. It is necessary to establish a quantitative method for determining IGF-1 in the cow milk. In this study, a LC-MSMS method based on Multiple Reaction Monitoring (MRM) is established for quantitative detection of IGF-1 in raw milk. Based on the analysis, the IGF concentration in the raw milk was determined to be 1.14pg/ul which is shown to be comparable to the value determined by ELISA method.

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