介白素-6 (Interleukin-6, IL-6) 在先前的文獻當中已被證實對於生物體的先天性免疫及後天免疫有著重要的影響。而介白素-6的功能表現需要在目標細胞與其受體結合後進行訊號傳遞，啟動如STAT-3分子的磷酸化，進而啟動相關下游基因表現。介白素-6主要與介白素-6受體 (Interleukin-6 receptor, IL-6R) 以及醣蛋白130 (glycoprotein 130, gp130) 所組成的複合受體結合，來起始訊息的調控。根據其訊號傳遞的過程中因與受體結合方式的不同分為trans-signaling 和classic-signaling pathway，分別使用兩種形式的介白素-6受體：soluble和 membrane-bound form 結合所致。這兩種訊息傳遞則發生於具有產生介白素-6受體的細胞（大多為免疫細胞）以及僅能表現醣蛋白130的細胞（大多數細胞皆能表現）目前在哺乳動物對於這兩種不同路徑所造成的免疫反應現象尚未有深入的探討。遑論硬骨魚類的相關研究。因此，本研究旨在探討在魚體中不同器官內，可以產生介白素-6受體以及醣蛋白130的細胞類型及其對應於不同免疫刺激環境的受體基因變化。以對未來探討魚類介白素-6 trans-signaling 和classic-signaling pathway的不同路徑研究提供基礎。

Interleukin-6 (IL-6) has been proved various effects on innate immunity and adaptive immunity in previous studies. Activation of IL-6 signaling needs combination between target cell and its receptors such as IL-6R and gp130. It’s known that IL-6, gp130 and IL-6R will aggregate as a complex which turns on the signaling transduction by phosphorylated molecules such as STAT-3. There are two signaling pathways: trans-signaling and classic-signaling pathway which due to combination between soluble or membrane-bound form of IL-6R. IL-6 receptor producing cells that were primarily derived from immune cells and gp130 only cells that almost expressed in all cells are participated in these two IL-6 signaling pathways. It’s still remained unclear between these two IL-6 signaling induced immune responses in mammalian, not to mention in teleost. In this study, the various cell types expressed il6r and gp130 among different immune organs will be figured out. And the mRNA level of bacterial induction last much longer than viral infection. The ligand boost may modulate IL-6 receptor expression. With lymphocyte cells aggregation, the IL-6 signaling will be reinforced if required. These results will be the cornerstones for studying teleost IL-6 trans-signaling and classic-signaling pathway mechanism in the future.

方苡帆，應用重組介白素-6於點帶石斑魚之抗病機轉研究，國立成功大學生物科技研究所碩士論文，2013。

陳信宏，點帶石斑魚介白素-6之基因轉殖、蛋白表現及功能分析，國立成功大學生物科技研究所碩士論文，2011。

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