在各種新興且多樣的再生能源中，生質能源具有相當的發展潛力。作為生質能源的原料之一，由於微藻富含高油脂、生長快速可短期內大量培養且不需佔用耕地面積、有效的固定二氧化碳、可產生許多高經濟價值產物，因此在生質能源的發展上一直佔有重要的地位。為了能夠讓以微藻為原料之生質能源發展更具有商業競爭力，如何快速分析微藻成分，作為篩選高含油量藻種之依據或是用於即時監控微藻生長的情形，進而最佳化培養的條件是相當重要的步驟之ㄧ。
本研究提出製作PDMS孔洞來輔助陽極氧化鋁薄膜抽氣過濾以得到微藻沉積物，並對其進行拉曼分析。實驗結果顯示，利用此方法可以得到較均勻分布的微藻沉積物，有效消除因自然揮發所產生的咖啡環效應。另外，整個分析時間縮短至5分鐘以內，且量測的微藻拉曼訊號也較一致。
另外，本實驗加入四氯金酸試劑利用微藻來還原生成奈米金粒子，透過奈米金粒子的形成以增顯微藻拉曼訊號，並探討四氯金酸濃度以及反應時間對微藻拉曼圖譜之影響。

In this study, rapid and direct Raman analysis of microalgae was proposed and demonstrated through microwells-assisted filtration using anodic aluminum oxide (AAO) membrane. Instead of evaporation, a droplet of algal solution was dispensed in the polydimethylsiloxane (PDMS) microwells, which was placed on top of the AAO membrane, followed by applying the vacuum. The algal cells deposited inside the microwells and were subsequently analyzed by Raman spectroscopy. The results showed that the algal cells were effectively concentrated and distributed quite uniformly inside the microwells, which led to uniform Raman signals among different algae pastes. Moreover, the time for preparing the algae paste was reduced to about 5 minutes. We also added HAuCl4 reagent to biosynthesize gold nanoparticles (AuNPs) and intended to enhance the Raman signals of the algal cells. The results showed that the lipid signals of the algal cells with biosynthesized gold nanoparticles were enhanced up to 20 times after algal cells being incubated in 10mM HAuCl4 for 15min. It was also found that carotenoids and chlorophyll may serve as reducing agents during reduction reaction.

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