奈米微粒已經被認為對肺部有潛在性的傷害。而超微細碳黑(ultrafine carbon black, ufCB) 為奈米微粒的一種，在目前的研究發現，超微細碳黑會造成肺部發炎反應、上皮細胞傷害與增加血管通透性，造成一些蛋白質變化量的改變。而利用蛋白質體的研究，可以大範圍的得知哪些蛋白質會受到ufCB暴露而影響其含量。因此，本篇研究的主要目的，即要探討小鼠受到超微細碳黑的暴露後，其蛋白質體的變化為何。在本研究中，將小鼠分為暴露組(6隻)與對照組(6隻)，並在暴露12小時後收集其肺沖提液進行分析。而在蛋白質體分析中，混合來自6個暴露組與混合來自6個對照組的肺沖提液，利用液相層析串聯式質譜儀(nano-HPLC-ESI-MS/MS)與資料庫的搜尋進行蛋白質鑑定，總共在兩組混合的肺沖提液中找到132個蛋白質。接著，為了更進一步得知蛋白質的變化，本研究利用液相層析質譜儀(nano-HPLC-ESI-MS)對12個個別樣本做比較性分析，找出因超微細碳黑暴露而有差異性的蛋白質。最後，有15個蛋白質受到暴露的影響而改變。這些蛋白質分別為group specific component, immunoglobulin heavy chain, MPR8 protein, zinc finger protein 36, surfactant associated protein D, kiningen 1, PREDICTED: solute carrier family 22 (organic cation transporter), member 15, SCRIB1(scribbled homolog 1), Sel1 (suppressor of lin-12) 1 homolog isoform a, Es 1 protein, hemopexin, OUT domain containing 7, profilaggrin, serine (or cysteine) proteinase inhibitor, clade A, member 3K, and 4631422O05Rik protein.而本研究的結果，也許可以提供一些資訊來了解由奈米微粒所誘發的肺部傷害與其機制。

Nanoparticles have been recognized as a potential hazard to the lung. Ultrafine carbon black (ufCB), one kind of nanoparticles, can cause pulmonary inflammation, epithelial damage, and increase of alveolar-capillary permeability in the lung, resulting in the alterations of protein expression. Proteomic research can help identify large-scale changed proteins associated with nanoparticles exposure. Therefore, the aim of this study is to investigate the proteome changes of mouse bronchoalveolar lavage fluid (BALF) associated with ufCB exposure by proteomic analysis. In this study, the mice were divided into an exposure (N = 6) and a control group (N = 6). The BALF samples were collected at 12hr postinstillation. The protein identification of two BALF samples pooled from 6 exposed mice and 6 controls were carried out by using nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry (nano-HPLC-ESI-MS/MS) and database searching. A total of 132 distinct proteins from these two groups were revealed. To further evaluate changes in protein levels, differential analyses were performed on those 12 individual BALF samples by using nano-HPLC-ESI-MS. The results revealed that 15 proteins exhibited significant changes after exposure. These proteins are group specific component, immunoglobulin heavy chain, MPR8 protein, zinc finger protein 36, surfactant associated protein D, kiningen 1, PREDICTED: solute carrier family 22 (organic cation transporter), member 15, SCRIB1(scribbled homolog 1), Sel1 (suppressor of lin-12) 1 homolog isoform a, Es 1 protein, hemopexin, OUT domain containing 7, profilaggrin, serine (or cysteine) proteinase inhibitor, clade A, member 3K, and 4631422O05Rik protein. The result obtained from this study may provide useful information to better understand the mechanisms involved in lung disorders induced by nanoparticle exposure.

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邱碧英(貿易發展委員會副執行秘書), 工業雜誌 民國91年6月
謝春成（數位基因公司執行長）, 經濟日報, 民91年8月