本研究使用微機電製程整合吸附電極和檢測電極發展出微型化離子吸附晶片以降低尿液中離子雜訊干擾，並應用在提升電性免疫鑑別法於尿液檢體上濃度之鑑別度。本研究策略利用電吸附原理吸附帶電離子，降低溶液內離子濃度。晶片設計上利用上下兩層吸附電極晶片與PDMS微流道晶片做緊密結合，並在後端設置檢測電極以即時檢測溶液阻抗值變化。本研究之離子吸附晶片在檢體總量150 L之下，當施加電壓由0 V提升至0.8 V時，檢體之溶液阻抗值最高提升13.5%，降低其檢體溶液內之離子濃度。運用本研究之離子吸附晶片處理含有促黃體生成素(Luteinizing hormone, LH)尿液檢體後，再由電性免疫鑑別法對三明治型免疫試劑進行濃度鑑別分析，其免疫反應偵測時間為5分鐘，檢體需求量為70 L，結果在低頻率時(100 Hz) 對比未經由離子吸附晶片處理之電性免疫鑑別分析，其免疫檢測阻抗電性訊號差值相對濃度變化之斜率提高44%，而其濃度對應阻抗值之線性度R2值提高40%。本研究將微型離子吸附晶片應用在電性免疫鑑別法中以降低檢體中離子電訊號干擾，達到濃度檢測、成本低廉、低檢體用量及提升電性鑑別度之目的，提供了檢測免疫分析上一個全新的方向與思維。

This study, a novel micro-electrode biochip integrated MEMS technology of adsorption electrodes and detection electrodes to reduce the interference of ions in urine. This applied to enhance the immune identification method in the concentration of the urine samples. The research strategy used electrosorption theory to reduce the ion concentration in solution. Chip design using two layers electrode chip with PDMS microfluidic chips, and set the detection electrodes in the back-end for real-time impedance detection. The study of ion adsorption chip in the total 150 μL samples below, when the applied voltage from 0 V to 0.8 V, samples of the solution resistance values up to 13.5% improved. The study used ion adsorption chip to process urine samples with luteinizing hormone (LH), and then identified by the electrical method of sandwich immune concentration discriminant analysis. The immune response detection time was 5 minutes with 70 μL samples. At low frequencies (100 Hz), the results of using ion adsorption chip compared with non-treated, the immune impedance detection slope of concentration increased 44%, and the concentration of the corresponding linear impedance degree R2 value increased 40%. In this study, the ion adsorption chip was used to improve the immune identification method, and to reduce the ion interference in samples. The concentration detection device to achieve the purpose of low cost, low-volume samples to identify and enhance the degree of impedance signals. It provided a new direction to improve the sensitivity of immunoassay method.

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