在本研究中，我們運用超長工作距離物鏡來作為取代傳統透鏡而作為光束聚焦裝置形成量測區，依此改良傳統傳統型態之雷射都卜勒測速儀而發展出全物鏡式雷射都卜勒測速儀，企圖在能達成縮小條紋間距同時，兼保有足夠的干涉條紋數，及在做微流元件流場量測時所須的彈性架設空間。
　　本系統經實施校正後，可獲得干涉條紋間距在1微米以下、干涉條紋數超過10條以上之量測體積；量測體積其條紋間距，可依彈性更換更高倍率物鏡而改變，進而提昇系統的空間解析度；同時也整合一新式簡易聲光調製手段暨訊號處理流程，可測出反向流場；此外我們也藉由校正設備實證兩種不同干涉光路：參考光路及雙光束干涉光路模式之訊號噪訊強度在比較時，雙光束模式噪訊會比較低的情形。
　　在本文中，我們用了電噴灑，及微管道流場作為外、內流場性能測定的對象，而全物鏡式雷射都卜勒測速儀也針對不同的量測對象，各別以架設適合之量測光路測得其速度分佈，和相對應之線性流速變化。在做電噴灑現象測速時，觀察該流場情形同時也得出發展全物鏡式雷射都卜勒測速儀於微流元件之流場量測應用在供給高流量時欲穩向電噴灑，必須同時控制表面張力及電場力的結論；而在管流量測中流速測定的結果，也顯示出因指示粒子的濃度不同，即使處於同一穩定流場速度下，粒子其反應速率也會有所差異的情形。
　　對於所發展出的全物鏡式雷射都卜勒測速系統，並搭配上自製的乳化粒子，本系統可能將用於量測電動液壓幫浦微流道晶片中，其脈衝不穩流之現象作測速研究；或是介電泳晶片中，作粒速量測並測定粒子特性。

　　In this study, we develop our whole objective lens-based laser Doppler anemometry by introducing the ultra long working distance objective lens instead of using conventional lens to focus beams and generate the measuring volume. This schemes to reduce the interference spacing and meantime to maintain sufficient pattern number. In addition, thus-designed instrument also provides adequate operation space for measuring flow field in micro-fluidic devices.
　　After calibrating our LDA system, we can discover that probe volume has spacing less than 1μm, and that over 10 pattern numbers has in it. This system can minimize its spacing by easily introducing higher multiplication objective lens and thus improving its spatial resolution. Simultaneously, the new acoustic modulation device and data processing program enable this whole objective lens based LDA to discriminate reversed flow field. Besides, we also set up two kinds of light path mode: reference and dual beam mode. While testing and comparing both mode’s signal quality, we reconfirm that the dual beam mode has less noise in sensing signal.
　　In this experiment, we generate an electro-spray and apply a micro channel flow as our testing bed. By establishing suitable light path mode for LDA, this system can find out the velocity profile of electro-spray, and it also obtains a linear changed speed condition in micro channel when fluid was driven by a syringe pump under several pumping rate, even in an over-seeded situation. Meanwhile, by observing the capillarity wetting of electro-spry which cause the sweeping of spray, we realize that it is necessary to control both surface tension and electric field at the same time to stabilize the spray direction if we apply larger flow rate to spray than usually used. 　　Finally, the micro channel flow measuring results tells us that the more dense seeding un flow will not accurately respond to the real flow speed in the micro channel.
　　In the future work, we prepare to use this tool with self-made seeding to investigate the pulse flow field in the electro-hydro dynamic pumping driven chip, or to measure velocity of particles and then estimate the particle properties in dielectrophoretic-force driven chip.

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