散尾葵(Dypsis lutescens (H.Wendl.) Beentje & J.Dransf.)為棕櫚科(Arecaceae)之植物並因其美麗的外觀在臺灣被廣泛種植為景觀植物。關於散尾葵種子成分的研究並不多，而其種子以農業廢棄物處理。在超氧陰離子產生以及彈性蛋白酶釋放的抑制試驗中，散尾葵之酒精萃取物具有顯著抗發炎活性。因此，本實驗目標為從散尾葵種子分離化合物，並尋找具有抗發炎活性之前驅物。
散尾葵種子之酒精萃取物首先以甲醇和己烷進行溶劑分劃，甲醇層再以正丁醇及水進行分劃，具有活性之正丁醇分劃層進一步利用一系列層析技術分離。純化後的化合物之結構由核磁共振儀以及質譜儀之圖譜鑑定，而抗發炎活性由超氧陰離子產生以及彈性蛋白酶釋放的抑制試驗評估。散尾葵種子萃取物中，一共分離出24個化合物，包括：2個indole glycoside成分(1-2)、9個benzenoid類成分(3-11)、4個phenylpropanoid類成分(12-15)、1個flavonoid類成分(16)、1個steroid類成分(17)、4個lipid類成分(18-21)以及glycerol (22)、 1-methoxy-2-propyl acetate (23)、dibutyl phthalate (24)。在HPLC分析中，結果顯示正丁醇層之重要的成分都有被分離出。化合物11和21在超氧陰離子產生抑制活性測試中具有顯著抗發炎活性，而彈性蛋白酶釋放的抑制試驗中，化合物21具有顯著抗發炎活性。

Dypsis lutescens (H.Wendl.) Beentje & J.Dransf. belongs to the Arecaceae family and is widely cultivated as ornamental plants in Taiwan because of its beautiful appearance. The components of D. lutescens seeds are rarely studied and the seeds become agricultural waste. In our preliminary screening of superoxide anion generation and elastase release inhibition assay, the ethanol extracts of the seeds of D. lutescens displayed significant anti-inflammatory activity. Therefore, this study aims to isolate the ingredients from the seeds of D. lutescens, and discover the precursors with anti-inflammatory activity.
The ethanol extract of the seeds of D. lutescens was partitioned with hexane and methanol. The methanol layer was further partitioned with n-butanol and water. The active n-butanol layer was separated by a series of chromatography techniques. The purified compounds were determined their chemical structures by NMR and mass spectrometry. In total, twenty-four compounds were isolated from the extracts of the seeds of D. lutescens, including two indole glycosides (1-2), together with nine benzenoids (3-11), four phenylpropanoids (12-15) , one flavonoid (16), one steroid (17), four lipids (18-21), glycerol (22), 1-methoxy-2-propyl acetate (23) and dibutyl phthalate (24). In HPLC analyses, the results shows that significant compounds in n-BuOH layer have been isolated. Compounds 11 and 21 show significant anti-inflammatory activity in superoxide anion generation inhibition assay, while compound 21 displays significant anti-inflammatory activity in elastase release inhibition assay.

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