螢光共振能量轉移(Fluorescence Resonance Energy Transfer, FRET) 的技術可用來檢測分子間的鍵結與交互作用。然而，於實際FRET檢測時，因待測樣品濃度較稀薄，造成FRET訊號通常很微弱，且需要較長時間才可檢測到訊號，所以本研究透過交流電荷動力學微機電系統來克服這些缺點。本研究使用的方法為利用有修飾streptavidin的奈米量子點(Quantum Dots, QD)作為螢光供體，捕捉目標分子免疫球蛋白G (Immunoglobulin G, IgG)標記Alexa 647作為螢光受體並以NHS biotin來進行修飾，再透過AC電場產生流體流動，使QD和IgG迅速集濃來加速兩者之間的鍵結速度，進而放大FRET訊號且可縮短分子檢測的時間，並提高FRET靈敏度達IgG-NHS biotin濃度至0.1 picoM.。
本文第三章，本研究使用四角金電極，為了選擇適當頻率進行FRET檢測， 藉由AC電場分別對QD和IgG做掃頻測試，實驗結果顯示，QD和IgG均會在開啟電場後，迅速的由電極四邊往中央被捕捉聚集，而此聚集主要是因使用的溶液為高導電度的buffer，產生導電度主導的交流電熱流(AC Electrothermal, ACET)流動，使QD和IgG迅速的被捕捉聚集在電極中央。
本文第四章，基於第三章成功利用ACET捕捉QD和IgG後，再以相對應電場條件進行FRET檢測，實驗結果顯示，在開啟電場後，立即在電極中央觀察到明顯的FRET訊號，且當亮度達峰值後，衰減下來的強度仍然明顯的高於背景強度並持續至少10分鐘。然而，也發現到在FRET訊號中，並不完全是因QD-IgG鍵結所形成的FRET訊號，其中可能還會包含些許QD的訊號。儘管如此，FRET靈敏度仍可達 IgG-NHS biotin濃度至0.1 picoM.。
本文第五章，最主要目的是要利用FRET強度之變化來確認QD-IgG的鍵結，當觀察到FRET訊號後，在電場保持開啟的狀態下注入酵素對IgG進行分解，實驗結果發現，在注入酵素後，FRET訊號會開始逐漸衰減，且與沒有加入酵素的強度相比明顯更弱，可證實在下探濃度後，長時間下的FRET訊號QD-IgG確實有成功鍵結。接著後續再對FRET訊號之淬滅，進一步利用動力學模式來進行分析，可能具有實現更精確的FRET在生物分子上探測的潛力。

Fluorescence Resonance Energy Transfer (FRET) is a robust technique for probing target molecules. However, because sample concentrations are typically very low, actual FRET signals are often very weak. In this thesis, we develop an AC electrokinetic microdevice to overcome this shortcoming. The approach involves the use of Quantum Dot(QD) as the FRET donor. It is used to probe the target Immunoglobulin G(IgG) labeled with the FRET acceptor Alexa647. We also identify that the observed trapping is due mainly to AC Electrothermal (ACET) flow by intense Joule heating in a high conductivity buffer solution used here. Using this approach, we find that not only can FRET signals be greatly amplified upon the application of an electric field, but also the detection sensitivity can be pushed to as low as 0.1 picoM.

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