自來水中臭味常是人民所詬病的水質問題，本研究主要可分為兩部分，一部分主要探討水庫土霉臭味之產生，利用95年澄清湖水庫底泥篩選出之放線菌，搭配2K因子設計培養水質條件(包括pH、碳、氮源種類、溫度)進行產臭實驗，以尋求主要影響產臭效率之因子;而另一部分為利用實驗室自行分離之Bacillus菌屬，以不同培養基組成與培養環境，探討實驗菌種對於水中臭味物質之降解效率，以求得降解效率之反應條件。
在初步產臭測試中，以S31及S32最具有明顯產臭能力，7天後2-MIB與Geosmin產生濃度分別可達715.7與4113.4ng/L，因此以此兩種菌進行後續批次產臭試驗，整體來看，Geosmin濃度遠低於2-MIB，而菌種S32菌產臭能力較S31為佳。經ANOVA軟體轉換效應分析後之結果發現，溫度、pH與(NH4)2CO3濃度及其互相結合之複合因子為主要影響因子。
藉由初步試驗發現，共代謝基質(乙醇)之添加的確大幅提高臭味物質降解效率，2-MIB之降解效率遠優於Geosmin，最佳降解期程為9天，菌種B-1與B-2可將100ng/L之2-MIB 分別降解至39.3ng/L及45.7ng/L，降解效率可達60.72%與54.33%，而Geosmin於初步測試試驗完成後，殘餘濃度仍高達90.2ng/L與95.4ng/L，降解效率不到10%。在生物降解批次試驗中，以共代謝之方式降解2-MIB，在pH=7.0、氮源種類為NH4NO3與培養溫度為35℃等培養條件下，降解效率可達80%以上。經由ANOVA軟體轉換效應分析後，pH值、氮源種類、溫度及其互相結合之複合因子為主要影響因子。在臭味物質降解方面，則以中溫中性之環境下效果成效較佳。

The odor is often one of the water quality questions that the people’s concern in the drinking water. This research can be divided into two parts mainly, one part main investigated the musty odor in the reservoir. We used the actinomyces which were screened in the sediment of Cheng-Chin reservoir in 2006 and collocated the 2K factor-design to simulate the water condition (include pH, carbon source, nitrogen source, and temperature) to further seek the main factors of influencing the odor-producing efficiency; The another part we selected the Bacillus that separated from our laboratory and enrichmented with different medium to investigate the odor-compounds degradation efficiency of this bacteria.
The results indicated that S31 and S32 has the significant efficiency of odor-producing. After 7 days, the concentration of 2-MIB and Geosmin could up to 715.7 and 4113.4ng/L, respectively.The concentration of Geosmin were all lower than the concentration of 2-MIB, and the bacterial S32 has better odor-producing ability than S31. After transferring with ANOVA software we found the temperature, pH and the concentration of ammonium carbonate were the main factors by influencing odor-producing efficiency.
In the biodegradated tests, we found the efficiency increased with increasing the concentration of cometabolism substrate and the degradation efficiency of 2-MIB is much better than Geosmin. The best degradation time was 9 days, and test bacterial B-1 and B-2 could degrade 2-MIB calculated to 39.3ng/L and 45.7ng/L with initial concentration were 100ng/L, respectively. The degradation efficiency are 60.72% and 54.33%. After the preliminary degradation test, the residual Geosmin concentration were 90.2ng/L and 95.4ng/L, and the degradation efficiency were less than 10%. In the biodegradation experiments, when the conditions were pH=7.0, nitrate source=NH4NO3, temperature=35℃, the degradation efficiency of 2-MIB can be up to over 80%. After transforming the effects via ANOVA software, we found pH value, nitrogen source, temperature were the main factors of biodegradation of odor compounds.

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許雅雯，「應用批次與連續流試驗探討實驗室分離之除臭菌株對水庫中臭味物質降解之能力」，國立成功大學環境工程研究所碩士論文，民國九十五年七月。

童淑珠，「水源中2-MIB與geosmin 鑑定與氧化之研究」，國立成功大學環境工程學系博士論文，民國九十五年六月。

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