原水中的霉味物質2-methylisoborneol(2-MIB)為藍綠菌生長的代謝物，是造成飲用水口感不佳的原因之一，雖研究證實此物質對人體無健康危害之風險，然嗅味問題會影響民眾對自來水的信心，因此解決水中臭味問題，為現代自來水處理中重要的一環。目前臺灣常用的傳統淨水處理程序，無法有效去除2-MIB，文獻指出生物處理可促進2-MIB的降解，因此若要發展及應用生物處理法，分離環境水體中的降解菌是一個重要的步驟。
本研究從臺灣具有高濃度2-MIB之水庫中，成功分離出其中三株對2-MIB標準品具有良好降解能力之降解菌，根據16S rRNA的定序結果判斷分別是Methylobacterium sp. LT, Methylobacterium sp. PA以及Methylophilus sp.，並比較三者在批次反應器中，對2-MIB的降解特性及降解效率。三者於高濃度(20 µg/L)的形況下，都可在一周內去除70%至80%的2-MIB，顯示生物降解在處理2-MIB臭味物質的潛力。然而未來若要用於水廠處理程序中，尚需以真實環境體系，包括水體的2-MIB、背景物質、及有機物等對降解菌之影響，此外可加強分離環境中具有降解能力之微生物，以找出效能好之降解菌。
本研究並建立了一套以PMA前處理，搭配即時定量聚合酶鏈鎖反應的方法，能快速定量反應器中活的細菌量的方法，可大量減少固態培養基的使用，並節省時間、降低利用濁度判讀產生的誤差。

2-methylisoborneol (2-MIB), a musty odor compound produced by cyanobacteria, is a major cause of odor problem in drinking water of Taiwan. Although they are not considered harmful to human health, these compounds may still cause the perception of drinking water and need to be controlled. Unfortunately, 2-MIB is difficult to be removed by conventional water treatment processes. Biodegradation of 2-MIB was found to be an effective process for water treatment in serval studies. In this study, three bacterial strains were isolated for the degradation of 2-MIB, from different reservoirs in Taiwan, and were determined as Methylobacterium sp. LT, Methylobacterium sp. PA and Methylophilus sp., based on 16S rRNA analysis. Based on the experimental results, the removal efficiencies of 2-MIB by the three strains in batch scale reactors were in the range of 70% to 80% in one week. A pseudo-first order reaction model was able to describe the degradation of 2-MIB at an initial concentration of 20 µg/L, with rate constants of from 0.23 to 0.32 d-1. The results of this study suggest that biodegradation of 2-MIB is a potential method to remove 2-MIB in drinking water treatment.

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