幾丁聚醣及透明質酸鈉皆為具有生物相容性之聚多醣體，廣泛應用於生醫材料領域。本研究即結合兩者材料，製備共聚微粒體做為藥物攜帶載體。其中，製程係結合乳化液滴交聯法與離子交聯法，並且以三聚磷酸鈉作為離子交聯劑，固化幾丁聚醣與透明質酸共聚微粒體結構。
本實驗設計透明質酸與三聚磷酸鈉之添加濃度為實驗參數，並經由統計分析兩者對粒徑及表面電荷之影響。接著，以雙氯芬酸鈉之標準藥物，作為微粒體之攜帶標的，探討幾丁聚醣與透明質酸共聚微粒體在藥物包埋率、負載率以及體外控制釋放之表現。
根據實驗結果，可得適當之幾丁聚醣與透明質酸共聚微粒體製備條件，並且能應用於藥物攜帶，而雙氯芬酸鈉即以物理吸附於微粒體。此外，在體外控制釋放實驗中發現，相較於未被包覆之雙氯芬酸鈉釋出率，幾丁聚醣與透明質酸共聚微粒體具有緩釋藥物能力。

Chitosan (CS) and sodium hyaluronate (HA) have been applied in the field of biomedical and pharmaceutical science because of the excellent biocompatibility, biodegradability, and bioactivity. The objective of this study aims to prepare microparticles composed of two kinds of materials, and intended for the drug delivery carriers. Therefore, we fabricated CS-HA microparticles by emulsion-droplet coalescence method and ionic gelification. Sodium tripolyphosphate (TPP) acted as ion cross-linker.
In this research, CS-HA microparticles are prepared with varied HA and TPP concentration. The controlled drug release system was investigated using the poorly soluble drug, diclofenac sodium (DFS), as a model drug. Characteristics of CS-HA microparticles including mean particle size and zeta potential are evaluated, and drug release tests were conducted.
Suitable conditions for fabricating CS-HA microparticles are presented. The model drug, DFS, was physically loaded into microparticles. The release rate of DFS from the CS-HA microparticles has been found to be slower in comparison to the DFS powder.

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